Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5563 | 3' | -55 | NC_001806.1 | + | 33657 | 0.67 | 0.902131 |
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Target: 5'- gGGGGUgucGCGGGccgUCUGCuGGC-CCGc -3' miRNA: 3'- -CCUCGa--CGCCCuaaAGAUG-CCGaGGC- -5' |
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| 5563 | 3' | -55 | NC_001806.1 | + | 26163 | 0.67 | 0.920082 |
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Target: 5'- gGGAGgaGCGGGGggaggaGCGGCcagacgCCGa -3' miRNA: 3'- -CCUCgaCGCCCUaaaga-UGCCGa-----GGC- -5' |
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| 5563 | 3' | -55 | NC_001806.1 | + | 25636 | 0.66 | 0.940673 |
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Target: 5'- uGGGGCUG-GGGAgggCUGgGGCUg-- -3' miRNA: 3'- -CCUCGACgCCCUaaaGAUgCCGAggc -5' |
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| 5563 | 3' | -55 | NC_001806.1 | + | 24136 | 0.68 | 0.888991 |
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Target: 5'- cGGGGCcGUGGaGUUUCUGgGGCUgCu -3' miRNA: 3'- -CCUCGaCGCCcUAAAGAUgCCGAgGc -5' |
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| 5563 | 3' | -55 | NC_001806.1 | + | 21764 | 0.66 | 0.940673 |
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Target: 5'- cGGGCcgucggGCGGGGUccgUCgagcGCGGCgCCGg -3' miRNA: 3'- cCUCGa-----CGCCCUAa--AGa---UGCCGaGGC- -5' |
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| 5563 | 3' | -55 | NC_001806.1 | + | 20727 | 0.7 | 0.783499 |
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Target: 5'- cGGGGggGCGGGGggccgGCGGcCUCCGc -3' miRNA: 3'- -CCUCgaCGCCCUaaagaUGCC-GAGGC- -5' |
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| 5563 | 3' | -55 | NC_001806.1 | + | 16156 | 0.71 | 0.733867 |
|
Target: 5'- aGGAGCUGcCGGGAgaccgcugcgaUCUGCgGGCgUCCc -3' miRNA: 3'- -CCUCGAC-GCCCUaa---------AGAUG-CCG-AGGc -5' |
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| 5563 | 3' | -55 | NC_001806.1 | + | 2764 | 0.67 | 0.902131 |
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Target: 5'- cGGGGCgcggGCGGGccugCgccgcgGCGGC-CCGg -3' miRNA: 3'- -CCUCGa---CGCCCuaaaGa-----UGCCGaGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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