Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5566 | 5' | -50.6 | NC_001806.1 | + | 136279 | 0.72 | 0.899348 |
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Target: 5'- cGCCccgGCgGUGGUUGUAguagauaUACACG-CGGUCg -3' miRNA: 3'- -UGG---CG-CACCAAUAU-------AUGUGCuGCCAG- -5' |
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| 5566 | 5' | -50.6 | NC_001806.1 | + | 121996 | 0.72 | 0.900011 |
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Target: 5'- cCCGCGUcuGGUUGUGUGugUAUGugGGUg -3' miRNA: 3'- uGGCGCA--CCAAUAUAU--GUGCugCCAg -5' |
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| 5566 | 5' | -50.6 | NC_001806.1 | + | 90313 | 0.68 | 0.976597 |
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Target: 5'- gGCCGUGgucgugGGUUcuc-GCACGACGGg- -3' miRNA: 3'- -UGGCGCa-----CCAAuauaUGUGCUGCCag -5' |
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| 5566 | 5' | -50.6 | NC_001806.1 | + | 63184 | 0.68 | 0.976597 |
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Target: 5'- uGCCGCG-GGUgcacugGgGCGugGGUUg -3' miRNA: 3'- -UGGCGCaCCAauaua-UgUGCugCCAG- -5' |
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| 5566 | 5' | -50.6 | NC_001806.1 | + | 104501 | 0.68 | 0.976597 |
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Target: 5'- uACUGCG-GGUU-UAUAUA-GACGGUCc -3' miRNA: 3'- -UGGCGCaCCAAuAUAUGUgCUGCCAG- -5' |
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| 5566 | 5' | -50.6 | NC_001806.1 | + | 148176 | 0.66 | 0.996364 |
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Target: 5'- uCUGCGUGGggGg--GCGCGGgGcGUCc -3' miRNA: 3'- uGGCGCACCaaUauaUGUGCUgC-CAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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