Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5566 | 5' | -50.6 | NC_001806.1 | + | 122444 | 0.74 | 0.818653 |
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Target: 5'- uCCGCGUGGUccccgaggaGCACGGCGGcCa -3' miRNA: 3'- uGGCGCACCAauaua----UGUGCUGCCaG- -5' |
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| 5566 | 5' | -50.6 | NC_001806.1 | + | 127515 | 0.67 | 0.988463 |
|
Target: 5'- aACgGCGgacGGUgcUGUACACGG-GGUCc -3' miRNA: 3'- -UGgCGCa--CCAauAUAUGUGCUgCCAG- -5' |
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| 5566 | 5' | -50.6 | NC_001806.1 | + | 129896 | 1.11 | 0.007936 |
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Target: 5'- uACCGCGUGGUUAUAUACACGACGGUCg -3' miRNA: 3'- -UGGCGCACCAAUAUAUGUGCUGCCAG- -5' |
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| 5566 | 5' | -50.6 | NC_001806.1 | + | 136279 | 0.72 | 0.899348 |
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Target: 5'- cGCCccgGCgGUGGUUGUAguagauaUACACG-CGGUCg -3' miRNA: 3'- -UGG---CG-CACCAAUAU-------AUGUGCuGCCAG- -5' |
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| 5566 | 5' | -50.6 | NC_001806.1 | + | 144800 | 0.66 | 0.99606 |
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Target: 5'- gGCCGCGUacggcccgggacgagGGgcccccgacCGCGGCGGUCc -3' miRNA: 3'- -UGGCGCA---------------CCaauauau--GUGCUGCCAG- -5' |
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| 5566 | 5' | -50.6 | NC_001806.1 | + | 148176 | 0.66 | 0.996364 |
|
Target: 5'- uCUGCGUGGggGg--GCGCGGgGcGUCc -3' miRNA: 3'- uGGCGCACCaaUauaUGUGCUgC-CAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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