Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5566 | 5' | -50.6 | NC_001806.1 | + | 37740 | 0.75 | 0.756376 |
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Target: 5'- aACCGCGUGGUg----GCGuCGAUGGUg -3' miRNA: 3'- -UGGCGCACCAauauaUGU-GCUGCCAg -5' |
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| 5566 | 5' | -50.6 | NC_001806.1 | + | 23958 | 0.66 | 0.993304 |
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Target: 5'- gGCCGCcggaGUGGUccgccgaGCGCGGCGGg- -3' miRNA: 3'- -UGGCG----CACCAauaua--UGUGCUGCCag -5' |
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| 5566 | 5' | -50.6 | NC_001806.1 | + | 13256 | 0.67 | 0.989872 |
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Target: 5'- aGCCGCa-GGUac-GUGC-CGGCGGUCu -3' miRNA: 3'- -UGGCGcaCCAauaUAUGuGCUGCCAG- -5' |
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| 5566 | 5' | -50.6 | NC_001806.1 | + | 9762 | 0.72 | 0.871578 |
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Target: 5'- cGCCGUG-GGUUGgggGCGCGugGGg- -3' miRNA: 3'- -UGGCGCaCCAAUauaUGUGCugCCag -5' |
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| 5566 | 5' | -50.6 | NC_001806.1 | + | 4018 | 0.66 | 0.995022 |
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Target: 5'- aGCCGCGUgaucaGGgcGUAcugcUGCGCGGCG-UCg -3' miRNA: 3'- -UGGCGCA-----CCaaUAU----AUGUGCUGCcAG- -5' |
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| 5566 | 5' | -50.6 | NC_001806.1 | + | 3539 | 0.67 | 0.986907 |
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Target: 5'- cCCGCG-GGggAggcggGCGCGGCGGa- -3' miRNA: 3'- uGGCGCaCCaaUaua--UGUGCUGCCag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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