Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5568 | 5' | -49.6 | NC_001806.1 | + | 127184 | 0.67 | 0.993735 |
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Target: 5'- cGUGCccCCGGAUauGCGCGGGGUuacGCGGGc -3' miRNA: 3'- uUAUGu-GGUCUG--UGCGUUUCA---CGUCC- -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 128395 | 1.1 | 0.009561 |
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Target: 5'- aAAUACACCAGACACGCAAAGUGCAGGa -3' miRNA: 3'- -UUAUGUGGUCUGUGCGUUUCACGUCC- -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 132969 | 0.68 | 0.983889 |
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Target: 5'- --aAgACUGGACGCGCu-GGUGguGGc -3' miRNA: 3'- uuaUgUGGUCUGUGCGuuUCACguCC- -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 134494 | 0.67 | 0.990381 |
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Target: 5'- --cACGCCAGAaccUugGCAAAGgucGaCGGGg -3' miRNA: 3'- uuaUGUGGUCU---GugCGUUUCa--C-GUCC- -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 138403 | 0.66 | 0.994532 |
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Target: 5'- --gACGCgGGGCACaugcgcuGCGAGGUGCAc- -3' miRNA: 3'- uuaUGUGgUCUGUG-------CGUUUCACGUcc -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 140131 | 0.67 | 0.990381 |
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Target: 5'- --gACGCCAGGCGCgGCAcacGGG-GUGGGc -3' miRNA: 3'- uuaUGUGGUCUGUG-CGU---UUCaCGUCC- -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 148601 | 0.66 | 0.994615 |
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Target: 5'- aGGUACACCAGcUugGCGuuGcacagcggGCAGGu -3' miRNA: 3'- -UUAUGUGGUCuGugCGUuuCa-------CGUCC- -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 151069 | 0.66 | 0.994615 |
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Target: 5'- --cGCGCgGGGguCGCGGGGgucGCGGGg -3' miRNA: 3'- uuaUGUGgUCUguGCGUUUCa--CGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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