Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5568 | 5' | -49.6 | NC_001806.1 | + | 100386 | 0.66 | 0.996077 |
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Target: 5'- ---uCGCCGGGCgGCGCGGGG-GcCGGGg -3' miRNA: 3'- uuauGUGGUCUG-UGCGUUUCaC-GUCC- -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 95309 | 0.66 | 0.996077 |
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Target: 5'- --cGCGgCGGACGCGCccAAGGgcggcGCGGGc -3' miRNA: 3'- uuaUGUgGUCUGUGCG--UUUCa----CGUCC- -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 71360 | 0.66 | 0.996077 |
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Target: 5'- --cACGCCAGGCGgacCGCGAGGggGCGc- -3' miRNA: 3'- uuaUGUGGUCUGU---GCGUUUCa-CGUcc -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 63123 | 0.66 | 0.996077 |
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Target: 5'- --aGCuuCAGGCAgGCcguGUGCAGGc -3' miRNA: 3'- uuaUGugGUCUGUgCGuuuCACGUCC- -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 51753 | 0.66 | 0.996077 |
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Target: 5'- --gGCGCCAaagcugcuGGCGCGCAccccguucAAGaGCGGGg -3' miRNA: 3'- uuaUGUGGU--------CUGUGCGU--------UUCaCGUCC- -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 69573 | 0.66 | 0.996676 |
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Target: 5'- --gGCGCCAGcgagcACACGCAcGG-GCGGc -3' miRNA: 3'- uuaUGUGGUC-----UGUGCGUuUCaCGUCc -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 76845 | 0.66 | 0.996676 |
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Target: 5'- -cUACGCgacaGGGCGCGCGcc-UGCAGGc -3' miRNA: 3'- uuAUGUGg---UCUGUGCGUuucACGUCC- -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 125832 | 0.66 | 0.997197 |
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Target: 5'- --gGCGCCuGACGCGCc----GCGGGc -3' miRNA: 3'- uuaUGUGGuCUGUGCGuuucaCGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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