Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5568 | 5' | -49.6 | NC_001806.1 | + | 22632 | 0.71 | 0.924057 |
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Target: 5'- --cGCACCGGGCgaGCGCGcGGUGCccgccGGGu -3' miRNA: 3'- uuaUGUGGUCUG--UGCGUuUCACG-----UCC- -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 76723 | 0.72 | 0.911999 |
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Target: 5'- uGGUGgACCAGACggagaaggcGCGCGAGcucgacGUGCAGGc -3' miRNA: 3'- -UUAUgUGGUCUG---------UGCGUUU------CACGUCC- -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 122645 | 0.73 | 0.852641 |
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Target: 5'- aAGUGCACCAG-CugGCGAaaggcuucggagcGGUGgAGGg -3' miRNA: 3'- -UUAUGUGGUCuGugCGUU-------------UCACgUCC- -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 104004 | 0.74 | 0.827648 |
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Target: 5'- --cAC-CCGGAgGCGCGAGGgacUGCAGGa -3' miRNA: 3'- uuaUGuGGUCUgUGCGUUUC---ACGUCC- -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 59361 | 0.75 | 0.790481 |
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Target: 5'- --cGCACCGGcGCAUGCAGGGUGCu-- -3' miRNA: 3'- uuaUGUGGUC-UGUGCGUUUCACGucc -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 57306 | 0.75 | 0.780765 |
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Target: 5'- --cGCGCCAGggcccccacGCACGCGAGGUGUGGc -3' miRNA: 3'- uuaUGUGGUC---------UGUGCGUUUCACGUCc -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 102520 | 0.78 | 0.63399 |
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Target: 5'- --cGCgGCCAGGCACGCcAGGUaGCGGGg -3' miRNA: 3'- uuaUG-UGGUCUGUGCGuUUCA-CGUCC- -5' |
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| 5568 | 5' | -49.6 | NC_001806.1 | + | 128395 | 1.1 | 0.009561 |
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Target: 5'- aAAUACACCAGACACGCAAAGUGCAGGa -3' miRNA: 3'- -UUAUGUGGUCUGUGCGUUUCACGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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