Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5570 | 3' | -58.2 | NC_001806.1 | + | 110201 | 0.67 | 0.780477 |
Target: 5'- cGUCCUCGUgcgUGCCGcACG-CGGuGGGGGc -3' miRNA: 3'- -CAGGAGUA---GUGGC-UGCuGCC-CCUCCa -5' |
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5570 | 3' | -58.2 | NC_001806.1 | + | 127091 | 1.07 | 0.002875 |
Target: 5'- cGUCCUCAUCACCGACGACGGGGAGGUc -3' miRNA: 3'- -CAGGAGUAGUGGCUGCUGCCCCUCCA- -5' |
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5570 | 3' | -58.2 | NC_001806.1 | + | 141787 | 0.66 | 0.839571 |
Target: 5'- cUCUUCGgucuggCACCGACGAuuauauuCGGccaGGAGGUg -3' miRNA: 3'- cAGGAGUa-----GUGGCUGCU-------GCC---CCUCCA- -5' |
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5570 | 3' | -58.2 | NC_001806.1 | + | 151318 | 0.68 | 0.733715 |
Target: 5'- -gCCUCuggCGCCGGCucgGGCGGGGGGc- -3' miRNA: 3'- caGGAGua-GUGGCUG---CUGCCCCUCca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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