miRNA: 3'- caGGAGUa-GUGg-CUGCUG-CCCCUCCa -5'
miRNA: 3'- cAGGaGUAGUGG-CUGCUGCcCCUCCa -5'
miRNA: 3'- cAGG-AGUAGUGGCU-----GCUGCc----CCUCCA- -5'
miRNA: 3'- caGGAG--------UA-GUgGCUGCUGCCCCUCCa -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5570 | 3' | -58.2 | NC_001806.1 | + | 90519 | 0.69 | 0.654943 |
|
Target: 5'- -aCCUCAaaCACgaGACGACgGGGGAGGc -3' miRNA: 3'- caGGAGUa-GUGg-CUGCUG-CCCCUCCa -5' |
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| 5570 | 3' | -58.2 | NC_001806.1 | + | 100380 | 0.69 | 0.684839 |
|
Target: 5'- cUCC-CGUCGCCgGGCGGCGcGGGGGc -3' miRNA: 3'- cAGGaGUAGUGG-CUGCUGCcCCUCCa -5' |
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| 5570 | 3' | -58.2 | NC_001806.1 | + | 70618 | 0.68 | 0.733715 |
|
Target: 5'- uUCCgUCAUCACCGAaaccaCGGCGcaccuGGGGGUg -3' miRNA: 3'- cAGG-AGUAGUGGCU-----GCUGCc----CCUCCA- -5' |
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| 5570 | 3' | -58.2 | NC_001806.1 | + | 99559 | 0.66 | 0.848259 |
|
Target: 5'- -gCCUCcgguugggAUcCGgUGGCGGCGGGGGGGa -3' miRNA: 3'- caGGAG--------UA-GUgGCUGCUGCCCCUCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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