Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5570 | 3' | -58.2 | NC_001806.1 | + | 73124 | 0.67 | 0.807072 |
Target: 5'- -gCCg-GUCGCCGA-GGCGGGcGAGGa -3' miRNA: 3'- caGGagUAGUGGCUgCUGCCC-CUCCa -5' |
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5570 | 3' | -58.2 | NC_001806.1 | + | 5141 | 0.66 | 0.815644 |
Target: 5'- cUCCgacgcCAUCGCCGAUG-CGGGGcgauccuccGGGg -3' miRNA: 3'- cAGGa----GUAGUGGCUGCuGCCCC---------UCCa -5' |
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5570 | 3' | -58.2 | NC_001806.1 | + | 141787 | 0.66 | 0.839571 |
Target: 5'- cUCUUCGgucuggCACCGACGAuuauauuCGGccaGGAGGUg -3' miRNA: 3'- cAGGAGUa-----GUGGCUGCU-------GCC---CCUCCA- -5' |
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5570 | 3' | -58.2 | NC_001806.1 | + | 99559 | 0.66 | 0.848259 |
Target: 5'- -gCCUCcgguugggAUcCGgUGGCGGCGGGGGGGa -3' miRNA: 3'- caGGAG--------UA-GUgGCUGCUGCCCCUCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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