Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5570 | 5' | -54.1 | NC_001806.1 | + | 90281 | 0.67 | 0.933841 |
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Target: 5'- cUGACCGUggagaGCGGCUUUgaggccaacguggccGugGUCGUGg -3' miRNA: 3'- -ACUGGCGgg---UGCUGAAA---------------CugCAGCAC- -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 89752 | 0.67 | 0.936812 |
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Target: 5'- cGGCCacGCCCAcCGGCUgaUGACGcgcggggCGUGg -3' miRNA: 3'- aCUGG--CGGGU-GCUGAa-ACUGCa------GCAC- -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 139843 | 0.67 | 0.936812 |
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Target: 5'- gGACCGCCUGCcGCgcggaGGCGUUGUu -3' miRNA: 3'- aCUGGCGGGUGcUGaaa--CUGCAGCAc -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 42017 | 0.67 | 0.946093 |
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Target: 5'- -uGCUGgCCGCGGggUUGGgGUCGUGg -3' miRNA: 3'- acUGGCgGGUGCUgaAACUgCAGCAC- -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 43485 | 0.66 | 0.950378 |
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Target: 5'- aGGCC-CCCACGAUc--GACGgggcCGUGg -3' miRNA: 3'- aCUGGcGGGUGCUGaaaCUGCa---GCAC- -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 30302 | 0.66 | 0.950378 |
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Target: 5'- cGGCCGCCCGCcccgcgGACgc--GCGcCGUGu -3' miRNA: 3'- aCUGGCGGGUG------CUGaaacUGCaGCAC- -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 101911 | 0.66 | 0.950378 |
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Target: 5'- cGGCCGCCC-CGcCUg-GGCGUCa-- -3' miRNA: 3'- aCUGGCGGGuGCuGAaaCUGCAGcac -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 52875 | 0.66 | 0.958251 |
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Target: 5'- cGACCGCCgGCGGC---GACGUg--- -3' miRNA: 3'- aCUGGCGGgUGCUGaaaCUGCAgcac -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 132834 | 0.66 | 0.961847 |
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Target: 5'- aGAcCCGCCCGCGGC-----CGUCGg- -3' miRNA: 3'- aCU-GGCGGGUGCUGaaacuGCAGCac -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 91698 | 0.66 | 0.965222 |
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Target: 5'- -cGCCGCCCGCGGagccaucGGCGUCu-- -3' miRNA: 3'- acUGGCGGGUGCUgaaa---CUGCAGcac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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