Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5570 | 5' | -54.1 | NC_001806.1 | + | 35774 | 0.68 | 0.909399 |
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Target: 5'- -aGCCGUCCGgGGCUUUGGCGccCGa- -3' miRNA: 3'- acUGGCGGGUgCUGAAACUGCa-GCac -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 30302 | 0.66 | 0.950378 |
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Target: 5'- cGGCCGCCCGCcccgcgGACgc--GCGcCGUGu -3' miRNA: 3'- aCUGGCGGGUG------CUGaaacUGCaGCAC- -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 28682 | 0.67 | 0.931812 |
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Target: 5'- -aGCCGCCCGCcauAUUUggggGACGcCGUGg -3' miRNA: 3'- acUGGCGGGUGc--UGAAa---CUGCaGCAC- -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 26957 | 0.68 | 0.909399 |
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Target: 5'- cGGCCGCCCGCcuGGCgcgccGCGgcUCGUGg -3' miRNA: 3'- aCUGGCGGGUG--CUGaaac-UGC--AGCAC- -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 21094 | 0.73 | 0.686702 |
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Target: 5'- gGAUCGCCCcgcauCGGCgaUGGCGUCGg- -3' miRNA: 3'- aCUGGCGGGu----GCUGaaACUGCAGCac -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 4628 | 0.68 | 0.909399 |
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Target: 5'- aUGGCCGCCaC-CGGCcgUGACGaCGUc -3' miRNA: 3'- -ACUGGCGG-GuGCUGaaACUGCaGCAc -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 4493 | 0.67 | 0.92657 |
|
Target: 5'- cGACgGCCCGCGcCUc--GCGUgCGUGg -3' miRNA: 3'- aCUGgCGGGUGCuGAaacUGCA-GCAC- -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 4392 | 0.68 | 0.903196 |
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Target: 5'- -cGCCGCCCGgGGCUUgGGCG-CGg- -3' miRNA: 3'- acUGGCGGGUgCUGAAaCUGCaGCac -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 4290 | 0.7 | 0.837244 |
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Target: 5'- gGGCUGCCCggccgugaaGCGGCccgUGGCGUCGc- -3' miRNA: 3'- aCUGGCGGG---------UGCUGaa-ACUGCAGCac -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 3467 | 0.69 | 0.861153 |
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Target: 5'- cGGCCGgCCugGGCgcggcgcccGGCGUCGUc -3' miRNA: 3'- aCUGGCgGGugCUGaaa------CUGCAGCAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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