Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5580 | 5' | -58.5 | NC_001806.1 | + | 124259 | 0.71 | 0.503184 |
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Target: 5'- cGGCU-UCAucGCGCCCCUCGGGUUc -3' miRNA: 3'- uCUGAuAGUcuCGUGGGGGGCCCAGc -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 129654 | 0.67 | 0.786311 |
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Target: 5'- cAGGCUGcCcu-GCGCCCgCCCGGGcCGc -3' miRNA: 3'- -UCUGAUaGucuCGUGGG-GGGCCCaGC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 131360 | 0.69 | 0.65121 |
|
Target: 5'- -cGCUGUCGGAGCuCCCCaCCGaGGcCu -3' miRNA: 3'- ucUGAUAGUCUCGuGGGG-GGC-CCaGc -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 135874 | 0.74 | 0.355914 |
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Target: 5'- cGuCUAUCAG-GUGCCCCCCGGccucGUCGa -3' miRNA: 3'- uCuGAUAGUCuCGUGGGGGGCC----CAGC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 148413 | 0.69 | 0.621109 |
|
Target: 5'- uGGGCga-CAGGGCccucaccguguGCCCCCCcaGGGUCa -3' miRNA: 3'- -UCUGauaGUCUCG-----------UGGGGGG--CCCAGc -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 151241 | 0.67 | 0.777249 |
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Target: 5'- gGGAgggGUCAGcccCGCCCCCCGGGcCc -3' miRNA: 3'- -UCUga-UAGUCuc-GUGGGGGGCCCaGc -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 151517 | 0.68 | 0.709817 |
|
Target: 5'- gGGACUGUgCGGuugggacGGCGCCCgugggCCCGGG-CGg -3' miRNA: 3'- -UCUGAUA-GUC-------UCGUGGG-----GGGCCCaGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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