Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5580 | 5' | -58.5 | NC_001806.1 | + | 85967 | 0.7 | 0.601069 |
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Target: 5'- gGGGCUu--GGAcGCGCCUCCCGGGgggUCGg -3' miRNA: 3'- -UCUGAuagUCU-CGUGGGGGGCCC---AGC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 22884 | 0.7 | 0.611082 |
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Target: 5'- gGGGCUGgcCGGGGCccggcccgccaGCCCCCCGcGGcCGg -3' miRNA: 3'- -UCUGAUa-GUCUCG-----------UGGGGGGC-CCaGC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 148413 | 0.69 | 0.621109 |
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Target: 5'- uGGGCga-CAGGGCccucaccguguGCCCCCCcaGGGUCa -3' miRNA: 3'- -UCUGauaGUCUCG-----------UGGGGGG--CCCAGc -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 3515 | 0.69 | 0.64118 |
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Target: 5'- cGGGCggcGUCGGGGUcgucGCCCCCCGcGGgggaggCGg -3' miRNA: 3'- -UCUGa--UAGUCUCG----UGGGGGGC-CCa-----GC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 131360 | 0.69 | 0.65121 |
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Target: 5'- -cGCUGUCGGAGCuCCCCaCCGaGGcCu -3' miRNA: 3'- ucUGAUAGUCUCGuGGGG-GGC-CCaGc -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 107187 | 0.68 | 0.678193 |
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Target: 5'- uGGGCUAUCAGcuGGCcuucgugcuggauaGCCCCUCGGcGUaCGa -3' miRNA: 3'- -UCUGAUAGUC--UCG--------------UGGGGGGCC-CA-GC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 31966 | 0.66 | 0.821147 |
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Target: 5'- gGGGCgggaCGGGGCGCCCaaaaggGGGUCGg -3' miRNA: 3'- -UCUGaua-GUCUCGUGGGggg---CCCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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