Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5580 | 5' | -58.5 | NC_001806.1 | + | 21772 | 0.66 | 0.804028 |
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Target: 5'- cGGGCgggGUCcgucGAGCGCggcgccggcagCCCCCGGG-CGg -3' miRNA: 3'- -UCUGa--UAGu---CUCGUG-----------GGGGGCCCaGC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 63378 | 0.66 | 0.804028 |
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Target: 5'- cAGAg-GUUGGAGCcggcgauggccGCCCCCUGGGUg- -3' miRNA: 3'- -UCUgaUAGUCUCG-----------UGGGGGGCCCAgc -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 56690 | 0.66 | 0.804028 |
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Target: 5'- gGGACg---GGGGC-CCgggaaCCCCGGGUCGc -3' miRNA: 3'- -UCUGauagUCUCGuGG-----GGGGCCCAGC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 17165 | 0.66 | 0.812666 |
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Target: 5'- -cGCUccaAGGGC-CCCCCCGGGaaggCGg -3' miRNA: 3'- ucUGAuagUCUCGuGGGGGGCCCa---GC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 4147 | 0.66 | 0.813521 |
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Target: 5'- cGGCUGUCGcccaggccgccguacAGCACCcgCCCCGGGggCGg -3' miRNA: 3'- uCUGAUAGUc--------------UCGUGG--GGGGCCCa-GC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 90487 | 0.66 | 0.821147 |
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Target: 5'- uGGCUuuUCGGAcuaCACCCCCCGGccCGg -3' miRNA: 3'- uCUGAu-AGUCUc--GUGGGGGGCCcaGC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 31966 | 0.66 | 0.821147 |
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Target: 5'- gGGGCgggaCGGGGCGCCCaaaaggGGGUCGg -3' miRNA: 3'- -UCUGaua-GUCUCGUGGGggg---CCCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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