Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5580 | 5' | -58.5 | NC_001806.1 | + | 85967 | 0.7 | 0.601069 |
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Target: 5'- gGGGCUu--GGAcGCGCCUCCCGGGgggUCGg -3' miRNA: 3'- -UCUGAuagUCU-CGUGGGGGGCCC---AGC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 23546 | 0.7 | 0.581118 |
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Target: 5'- cGGCUggCGGcGGCAgCCCCCGGGg-- -3' miRNA: 3'- uCUGAuaGUC-UCGUgGGGGGCCCagc -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 74920 | 0.71 | 0.522298 |
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Target: 5'- gAGGCcGUUcggGGGGCGCCCCCUGaGGUUa -3' miRNA: 3'- -UCUGaUAG---UCUCGUGGGGGGC-CCAGc -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 124259 | 0.71 | 0.503184 |
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Target: 5'- cGGCU-UCAucGCGCCCCUCGGGUUc -3' miRNA: 3'- uCUGAuAGUcuCGUGGGGGGCCCAGc -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 115539 | 0.72 | 0.447832 |
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Target: 5'- cGACguaCAGuuuaAGCGCCCCCCGGGgugccgCGa -3' miRNA: 3'- uCUGauaGUC----UCGUGGGGGGCCCa-----GC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 135874 | 0.74 | 0.355914 |
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Target: 5'- cGuCUAUCAG-GUGCCCCCCGGccucGUCGa -3' miRNA: 3'- uCuGAUAGUCuCGUGGGGGGCC----CAGC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 123181 | 1.08 | 0.00215 |
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Target: 5'- gAGACUAUCAGAGCACCCCCCGGGUCGc -3' miRNA: 3'- -UCUGAUAGUCUCGUGGGGGGCCCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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