Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5592 | 5' | -53.3 | NC_001806.1 | + | 71755 | 0.67 | 0.945142 |
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Target: 5'- -gGAACGCGGGGgggaUCGCG-ACAuCGUGg -3' miRNA: 3'- caCUUGCGCCCUa---GGUGCaUGU-GCAU- -5' |
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| 5592 | 5' | -53.3 | NC_001806.1 | + | 99590 | 0.67 | 0.953693 |
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Target: 5'- -gGAACGCGGGcUCCGuCGguagagggGCGCGc- -3' miRNA: 3'- caCUUGCGCCCuAGGU-GCa-------UGUGCau -5' |
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| 5592 | 5' | -53.3 | NC_001806.1 | + | 83825 | 0.67 | 0.953693 |
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Target: 5'- gGUcGGCGCGGGAUCCG---GCACGc- -3' miRNA: 3'- -CAcUUGCGCCCUAGGUgcaUGUGCau -5' |
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| 5592 | 5' | -53.3 | NC_001806.1 | + | 70464 | 0.66 | 0.957612 |
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Target: 5'- -aGAGCGaCGGGcgCCGCcuGUAUGCGg- -3' miRNA: 3'- caCUUGC-GCCCuaGGUG--CAUGUGCau -5' |
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| 5592 | 5' | -53.3 | NC_001806.1 | + | 113884 | 0.66 | 0.961297 |
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Target: 5'- gGUuAGCGCGGGGggCCACGaccccgugUACGCGg- -3' miRNA: 3'- -CAcUUGCGCCCUa-GGUGC--------AUGUGCau -5' |
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| 5592 | 5' | -53.3 | NC_001806.1 | + | 52080 | 0.66 | 0.967989 |
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Target: 5'- cUGGAC-CGGGGUCCcaGCG-GCACGa- -3' miRNA: 3'- cACUUGcGCCCUAGG--UGCaUGUGCau -5' |
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| 5592 | 5' | -53.3 | NC_001806.1 | + | 22827 | 0.66 | 0.967989 |
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Target: 5'- ----cCGCGGGAUCCugGagGCGCu-- -3' miRNA: 3'- cacuuGCGCCCUAGGugCa-UGUGcau -5' |
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| 5592 | 5' | -53.3 | NC_001806.1 | + | 25514 | 0.66 | 0.971007 |
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Target: 5'- -gGAGCGCGGGAcgcgCCGgGgaggGCugGg- -3' miRNA: 3'- caCUUGCGCCCUa---GGUgCa---UGugCau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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