Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 5658 | 5' | -55.9 | NC_001806.1 | + | 114352 | 0.66 | 0.912333 |
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Target: 5'- gGCGGCgccCGACGCGgGCGCCaACAc--- -3' miRNA: 3'- -CGCCG---GCUGUGCaUGCGGcUGUacaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 9199 | 0.66 | 0.912333 |
|
Target: 5'- cGCGGgUGugGCGaggGCGCgguCGACGUGg- -3' miRNA: 3'- -CGCCgGCugUGCa--UGCG---GCUGUACaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 2178 | 0.66 | 0.912333 |
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Target: 5'- aGC-GCCGACACGUcggggGCGCCGGu----- -3' miRNA: 3'- -CGcCGGCUGUGCA-----UGCGGCUguacaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 23051 | 0.66 | 0.900005 |
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Target: 5'- cGUGGCCGGCggcaGCG-AgGCCGcCGUGg- -3' miRNA: 3'- -CGCCGGCUG----UGCaUgCGGCuGUACaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 33304 | 0.66 | 0.899364 |
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Target: 5'- aGCGGCCGGCcgGCGcuuagggggaggaUGuCGCCGACGc--- -3' miRNA: 3'- -CGCCGGCUG--UGC-------------AU-GCGGCUGUacaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 84144 | 0.66 | 0.893492 |
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Target: 5'- cGCGG--GGCACGcACGCgCGACGUGg- -3' miRNA: 3'- -CGCCggCUGUGCaUGCG-GCUGUACaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 4307 | 0.66 | 0.893492 |
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Target: 5'- aGCGGcCCGugGCGUcGCGgCCGGCc---- -3' miRNA: 3'- -CGCC-GGCugUGCA-UGC-GGCUGuacaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 95506 | 0.66 | 0.923718 |
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Target: 5'- gGCGGCUGuACGCGgacCGCCuGACGa--- -3' miRNA: 3'- -CGCCGGC-UGUGCau-GCGG-CUGUacaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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