Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5747 | 5' | -53.6 | NC_001806.1 | + | 107530 | 0.66 | 0.959204 |
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Target: 5'- uUCGCCaUGCGcGGCCUcccUGCGcuuuGAUCUa -3' miRNA: 3'- gGGCGGcACGCaCCGGA---AUGU----UUAGA- -5' |
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| 5747 | 5' | -53.6 | NC_001806.1 | + | 110250 | 0.69 | 0.891144 |
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Target: 5'- cCCCGaCGUGCGUuGCacagGCAGGUCUc -3' miRNA: 3'- -GGGCgGCACGCAcCGgaa-UGUUUAGA- -5' |
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| 5747 | 5' | -53.6 | NC_001806.1 | + | 114937 | 0.74 | 0.634807 |
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Target: 5'- aCCGCCGU-CGUGGCCacgGCAAccGUCc -3' miRNA: 3'- gGGCGGCAcGCACCGGaa-UGUU--UAGa -5' |
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| 5747 | 5' | -53.6 | NC_001806.1 | + | 122532 | 0.68 | 0.91049 |
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Target: 5'- uCCgGCCGUGgGUGGCCa--------- -3' miRNA: 3'- -GGgCGGCACgCACCGGaauguuuaga -5' |
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| 5747 | 5' | -53.6 | NC_001806.1 | + | 127758 | 0.68 | 0.90428 |
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Target: 5'- gCUGCCGggUGCG-GGCCUgugGCGGccGUCUc -3' miRNA: 3'- gGGCGGC--ACGCaCCGGAa--UGUU--UAGA- -5' |
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| 5747 | 5' | -53.6 | NC_001806.1 | + | 135756 | 0.74 | 0.655528 |
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Target: 5'- aCgGCCGUGCGgaaGGCCUgGCGAAgCUg -3' miRNA: 3'- gGgCGGCACGCa--CCGGAaUGUUUaGA- -5' |
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| 5747 | 5' | -53.6 | NC_001806.1 | + | 139270 | 0.66 | 0.959204 |
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Target: 5'- cCCUGCCuuaCGUGGUCUUGCGGGa-- -3' miRNA: 3'- -GGGCGGcacGCACCGGAAUGUUUaga -5' |
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| 5747 | 5' | -53.6 | NC_001806.1 | + | 148504 | 0.68 | 0.90428 |
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Target: 5'- gCCCGCCcUGaCGgccuccucGGCCUccaUGCGGGUCUg -3' miRNA: 3'- -GGGCGGcAC-GCa-------CCGGA---AUGUUUAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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