Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5768 | 3' | -56 | NC_001806.1 | + | 120248 | 0.66 | 0.911303 |
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Target: 5'- -----uGCGCGCCgUCGAGGGgacCGCCCc -3' miRNA: 3'- cacaagUGCGUGG-AGCUCCU---GUGGGc -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 76854 | 0.66 | 0.911303 |
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Target: 5'- -aGggCGCGCGCCUgcAGGcgcucuucgACACCCGu -3' miRNA: 3'- caCaaGUGCGUGGAgcUCC---------UGUGGGC- -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 103039 | 0.66 | 0.917135 |
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Target: 5'- ---gUCuccaGCGCCUCcAGGGCGCCUGc -3' miRNA: 3'- cacaAGug--CGUGGAGcUCCUGUGGGC- -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 52066 | 0.66 | 0.917135 |
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Target: 5'- -cGUUCGCGCGCCaUCu-GGACcgggguCCCa -3' miRNA: 3'- caCAAGUGCGUGG-AGcuCCUGu-----GGGc -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 91658 | 0.66 | 0.917135 |
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Target: 5'- -cGcUCAUGCGCCUCc-GGGCGCgCCa -3' miRNA: 3'- caCaAGUGCGUGGAGcuCCUGUG-GGc -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 6066 | 0.66 | 0.922731 |
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Target: 5'- ----cCGCGCcgGCCcCGGGGGCggGCCCGg -3' miRNA: 3'- cacaaGUGCG--UGGaGCUCCUG--UGGGC- -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 70127 | 0.66 | 0.922731 |
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Target: 5'- cUGggCGCGCugaucacccuCCUCGAaccGGcCGCCCGg -3' miRNA: 3'- cACaaGUGCGu---------GGAGCU---CCuGUGGGC- -5' |
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| 5768 | 3' | -56 | NC_001806.1 | + | 150892 | 0.66 | 0.922731 |
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Target: 5'- -cGggCACGgGCCUCGGGccccaGGCacgGCCCGa -3' miRNA: 3'- caCaaGUGCgUGGAGCUC-----CUG---UGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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