Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5926 | 5' | -55.9 | NC_001806.1 | + | 151102 | 0.68 | 0.843689 |
Target: 5'- -gGGGUCGCGG-GggUCGCGggGGGc -3' miRNA: 3'- ggCCCAGUGCUaCuuAGCGCggUCCa -5' |
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5926 | 5' | -55.9 | NC_001806.1 | + | 25508 | 0.68 | 0.827348 |
Target: 5'- cCCGaGGgaGCGcgGGA-CGCGCCGGGg -3' miRNA: 3'- -GGC-CCagUGCuaCUUaGCGCGGUCCa -5' |
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5926 | 5' | -55.9 | NC_001806.1 | + | 52346 | 0.69 | 0.818913 |
Target: 5'- gCGGGcCGCGGUGGAca--GCCAGGa -3' miRNA: 3'- gGCCCaGUGCUACUUagcgCGGUCCa -5' |
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5926 | 5' | -55.9 | NC_001806.1 | + | 56709 | 0.69 | 0.783591 |
Target: 5'- cCCGGGUCGCucUGggUCcuccgggggGCGgCGGGa -3' miRNA: 3'- -GGCCCAGUGcuACuuAG---------CGCgGUCCa -5' |
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5926 | 5' | -55.9 | NC_001806.1 | + | 150981 | 0.7 | 0.74614 |
Target: 5'- gCGGGccCACGAgccgcggCGCGCCAGGc -3' miRNA: 3'- gGCCCa-GUGCUacuua--GCGCGGUCCa -5' |
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5926 | 5' | -55.9 | NC_001806.1 | + | 113168 | 0.76 | 0.43338 |
Target: 5'- gCGGGUC-CGAUGggUCGCggaagGCCAGa- -3' miRNA: 3'- gGCCCAGuGCUACuuAGCG-----CGGUCca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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