Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5971 | 5' | -52.6 | NC_001806.1 | + | 147722 | 0.66 | 0.974422 |
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Target: 5'- cUCGCACG-CACGgggcCA-CgGCCGCGCg -3' miRNA: 3'- cAGUGUGCaGUGCa---GUaGgUGGUGUG- -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 95095 | 0.66 | 0.974422 |
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Target: 5'- aUCGCGCGUU-CGggagCCACCugGCg -3' miRNA: 3'- cAGUGUGCAGuGCaguaGGUGGugUG- -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 103933 | 0.66 | 0.979366 |
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Target: 5'- gGUCACGCGgcugaCGCGgagCGUCCGuuGgGCg -3' miRNA: 3'- -CAGUGUGCa----GUGCa--GUAGGUggUgUG- -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 82617 | 0.66 | 0.981554 |
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Target: 5'- --aGCACG-CGCGUCAgCC-CCGCAg -3' miRNA: 3'- cagUGUGCaGUGCAGUaGGuGGUGUg -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 4317 | 0.66 | 0.981554 |
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Target: 5'- ----gGCGUCGCGgccggcCA-CCGCCGCGCg -3' miRNA: 3'- cagugUGCAGUGCa-----GUaGGUGGUGUG- -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 140877 | 0.65 | 0.983175 |
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Target: 5'- cGUCACGuagggccCGUCGguacagggcgggcCGUCAUCCG-CGCGCg -3' miRNA: 3'- -CAGUGU-------GCAGU-------------GCAGUAGGUgGUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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