Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5971 | 5' | -52.6 | NC_001806.1 | + | 2482 | 0.71 | 0.834562 |
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Target: 5'- gGUUAU-CGUCGuCGUCGccgCCGCCGCACg -3' miRNA: 3'- -CAGUGuGCAGU-GCAGUa--GGUGGUGUG- -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 136306 | 0.74 | 0.701117 |
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Target: 5'- -aCACGCGgu-CGUaGUCCGCCACGCg -3' miRNA: 3'- caGUGUGCaguGCAgUAGGUGGUGUG- -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 14837 | 0.75 | 0.638702 |
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Target: 5'- cGUCGCccGCGUCugGacCAaCCGCCACACa -3' miRNA: 3'- -CAGUG--UGCAGugCa-GUaGGUGGUGUG- -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 85756 | 0.77 | 0.513911 |
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Target: 5'- -cCGCGCGgcCACGUCGUCCgggggguGCCACACu -3' miRNA: 3'- caGUGUGCa-GUGCAGUAGG-------UGGUGUG- -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 60717 | 0.77 | 0.495091 |
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Target: 5'- -cCGCgACGcCACGUCAUCCACgACGCc -3' miRNA: 3'- caGUG-UGCaGUGCAGUAGGUGgUGUG- -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 438 | 1.09 | 0.005867 |
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Target: 5'- cGUCACACGUCACGUCAUCCACCACACc -3' miRNA: 3'- -CAGUGUGCAGUGCAGUAGGUGGUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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