Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5971 | 5' | -52.6 | NC_001806.1 | + | 110804 | 0.66 | 0.97165 |
|
Target: 5'- -gCAUAC-UCGCGUCuUCCGCCcCAUa -3' miRNA: 3'- caGUGUGcAGUGCAGuAGGUGGuGUG- -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 127635 | 0.68 | 0.946027 |
|
Target: 5'- cGUCG---GUUugGUCGUCCGCCACc- -3' miRNA: 3'- -CAGUgugCAGugCAGUAGGUGGUGug -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 136306 | 0.74 | 0.701117 |
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Target: 5'- -aCACGCGgu-CGUaGUCCGCCACGCg -3' miRNA: 3'- caGUGUGCaguGCAgUAGGUGGUGUG- -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 140877 | 0.65 | 0.983175 |
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Target: 5'- cGUCACGuagggccCGUCGguacagggcgggcCGUCAUCCG-CGCGCg -3' miRNA: 3'- -CAGUGU-------GCAGU-------------GCAGUAGGUgGUGUG- -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 147642 | 0.68 | 0.950391 |
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Target: 5'- -gCACACGgCGCG-CGUCCGCgGgGCg -3' miRNA: 3'- caGUGUGCaGUGCaGUAGGUGgUgUG- -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 147722 | 0.66 | 0.974422 |
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Target: 5'- cUCGCACG-CACGgggcCA-CgGCCGCGCg -3' miRNA: 3'- cAGUGUGCaGUGCa---GUaGgUGGUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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