Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5971 | 5' | -52.6 | NC_001806.1 | + | 5405 | 0.67 | 0.958397 |
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Target: 5'- gGUCcCGCGUCGgGUCGuggaUCCgugucggcaGCCGCGCu -3' miRNA: 3'- -CAGuGUGCAGUgCAGU----AGG---------UGGUGUG- -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 4317 | 0.66 | 0.981554 |
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Target: 5'- ----gGCGUCGCGgccggcCA-CCGCCGCGCg -3' miRNA: 3'- cagugUGCAGUGCa-----GUaGGUGGUGUG- -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 2482 | 0.71 | 0.834562 |
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Target: 5'- gGUUAU-CGUCGuCGUCGccgCCGCCGCACg -3' miRNA: 3'- -CAGUGuGCAGU-GCAGUa--GGUGGUGUG- -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 1869 | 0.68 | 0.936562 |
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Target: 5'- -gCGCGCGUgCGCGgCcUCCACgCGCGCg -3' miRNA: 3'- caGUGUGCA-GUGCaGuAGGUG-GUGUG- -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 1808 | 0.68 | 0.950391 |
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Target: 5'- -gCACGCGguagcgCACGUUG-CCGCCGCGg -3' miRNA: 3'- caGUGUGCa-----GUGCAGUaGGUGGUGUg -5' |
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| 5971 | 5' | -52.6 | NC_001806.1 | + | 438 | 1.09 | 0.005867 |
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Target: 5'- cGUCACACGUCACGUCAUCCACCACACc -3' miRNA: 3'- -CAGUGUGCAGUGCAGUAGGUGGUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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