Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5978 | 3' | -50.6 | NC_001806.1 | + | 93708 | 0.68 | 0.976796 |
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Target: 5'- gGGUUGGg----GGGUGGGGGAAccuAGGg -3' miRNA: 3'- gCCAGCUacgaaCCCACCCUUUU---UCC- -5' |
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| 5978 | 3' | -50.6 | NC_001806.1 | + | 103422 | 0.7 | 0.957983 |
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Target: 5'- gGGgacaaGAUGgg-GGGUGGGAuGAGGGg -3' miRNA: 3'- gCCag---CUACgaaCCCACCCUuUUUCC- -5' |
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| 5978 | 3' | -50.6 | NC_001806.1 | + | 110115 | 0.68 | 0.979198 |
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Target: 5'- aCGGUgGAUGUg-GGcGUGGGuauGGGGGa -3' miRNA: 3'- -GCCAgCUACGaaCC-CACCCuu-UUUCC- -5' |
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| 5978 | 3' | -50.6 | NC_001806.1 | + | 110704 | 0.72 | 0.88037 |
|
Target: 5'- uGGUCGAcgagGCcgccuucgGGG-GGGAGGAGGGa -3' miRNA: 3'- gCCAGCUa---CGaa------CCCaCCCUUUUUCC- -5' |
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| 5978 | 3' | -50.6 | NC_001806.1 | + | 122004 | 0.68 | 0.979198 |
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Target: 5'- uGGUUGuGUGUguaugUGGGUGGGu---GGGu -3' miRNA: 3'- gCCAGC-UACGa----ACCCACCCuuuuUCC- -5' |
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| 5978 | 3' | -50.6 | NC_001806.1 | + | 145518 | 0.7 | 0.940801 |
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Target: 5'- uGGcucUCuAUGCUUGGGUcuuacugccuGGGggGGGGGa -3' miRNA: 3'- gCC---AGcUACGAACCCA----------CCCuuUUUCC- -5' |
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| 5978 | 3' | -50.6 | NC_001806.1 | + | 145661 | 0.67 | 0.989929 |
|
Target: 5'- cCGGggUGAUcucuggcacGCggGGGUGGGAAGGgucGGGg -3' miRNA: 3'- -GCCa-GCUA---------CGaaCCCACCCUUUU---UCC- -5' |
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| 5978 | 3' | -50.6 | NC_001806.1 | + | 149854 | 0.66 | 0.995036 |
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Target: 5'- gGGUCGuAUGCggcuggaGGGUcGcGGAcgGAGGg -3' miRNA: 3'- gCCAGC-UACGaa-----CCCA-C-CCUuuUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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