Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5978 | 3' | -50.6 | NC_001806.1 | + | 15386 | 0.66 | 0.993331 |
Target: 5'- uGGUCugGAguuggGgUUGGGUGGuGGAGGAGa -3' miRNA: 3'- gCCAG--CUa----CgAACCCACC-CUUUUUCc -5' |
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5978 | 3' | -50.6 | NC_001806.1 | + | 31584 | 0.66 | 0.993331 |
Target: 5'- gCGGg-GAUGCgUGGGagugggggugcgUGGGAGugGGGg -3' miRNA: 3'- -GCCagCUACGaACCC------------ACCCUUuuUCC- -5' |
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5978 | 3' | -50.6 | NC_001806.1 | + | 32591 | 0.66 | 0.993235 |
Target: 5'- uGGUCGGacggGUaaguaacagagucUgacuaaGGGUGGGAGGGGGGg -3' miRNA: 3'- gCCAGCUa---CG-------------Aa-----CCCACCCUUUUUCC- -5' |
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5978 | 3' | -50.6 | NC_001806.1 | + | 34468 | 0.67 | 0.992319 |
Target: 5'- aGGagGAgaagGCggGGGgggGGGAGAcGGGg -3' miRNA: 3'- gCCagCUa---CGaaCCCa--CCCUUUuUCC- -5' |
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5978 | 3' | -50.6 | NC_001806.1 | + | 145661 | 0.67 | 0.989929 |
Target: 5'- cCGGggUGAUcucuggcacGCggGGGUGGGAAGGgucGGGg -3' miRNA: 3'- -GCCa-GCUA---------CGaaCCCACCCUUUU---UCC- -5' |
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5978 | 3' | -50.6 | NC_001806.1 | + | 38094 | 0.67 | 0.986994 |
Target: 5'- uGGUCGGUGg--GGGUuGGAGGcGGGc -3' miRNA: 3'- gCCAGCUACgaaCCCAcCCUUUuUCC- -5' |
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5978 | 3' | -50.6 | NC_001806.1 | + | 15298 | 0.67 | 0.986994 |
Target: 5'- uGGUCGGgcgugguaagGCUgaugGcGGUGGGAGAGucGGu -3' miRNA: 3'- gCCAGCUa---------CGAa---C-CCACCCUUUUu-CC- -5' |
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5978 | 3' | -50.6 | NC_001806.1 | + | 5295 | 1.12 | 0.006533 |
Target: 5'- cCGGUCGAUGCUUGGGUGGGAAAAAGGa -3' miRNA: 3'- -GCCAGCUACGAACCCACCCUUUUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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