Results 21 - 29 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5979 | 5' | -54.8 | NC_001806.1 | + | 38097 | 0.67 | 0.920243 |
Target: 5'- uCGGUG--GGGGUugGAGgcgggccaccuugcCCCCGUGCu -3' miRNA: 3'- -GCCAUuaCUCUAugCUC--------------GGGGCGCGc -5' |
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5979 | 5' | -54.8 | NC_001806.1 | + | 90162 | 0.67 | 0.927336 |
Target: 5'- cCGGgccccUGGGAUACGuGUacgCUCGCGCGu -3' miRNA: 3'- -GCCauu--ACUCUAUGCuCG---GGGCGCGC- -5' |
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5979 | 5' | -54.8 | NC_001806.1 | + | 38194 | 0.67 | 0.927336 |
Target: 5'- uGGU--UGAGGaucgUugGGGCCCUGcCGCu -3' miRNA: 3'- gCCAuuACUCU----AugCUCGGGGC-GCGc -5' |
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5979 | 5' | -54.8 | NC_001806.1 | + | 65626 | 0.67 | 0.927336 |
Target: 5'- uGGUGgccGUGGGcgagAgGGGCCCCG-GCGg -3' miRNA: 3'- gCCAU---UACUCua--UgCUCGGGGCgCGC- -5' |
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5979 | 5' | -54.8 | NC_001806.1 | + | 39205 | 0.66 | 0.932518 |
Target: 5'- uGcGUucuUGGGGgcCGGGCCCCGcCGCa -3' miRNA: 3'- gC-CAuu-ACUCUauGCUCGGGGC-GCGc -5' |
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5979 | 5' | -54.8 | NC_001806.1 | + | 89513 | 0.66 | 0.932518 |
Target: 5'- gCGGauccGGGcgGCGAGCUgCUGCGCGg -3' miRNA: 3'- -GCCauuaCUCuaUGCUCGG-GGCGCGC- -5' |
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5979 | 5' | -54.8 | NC_001806.1 | + | 62296 | 0.66 | 0.932518 |
Target: 5'- aCGGcgacgacAGUG-GcgGCGGGCCUgGCGCGg -3' miRNA: 3'- -GCCa------UUACuCuaUGCUCGGGgCGCGC- -5' |
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5979 | 5' | -54.8 | NC_001806.1 | + | 46891 | 0.66 | 0.937461 |
Target: 5'- gGGaAGUcacGAGGUAgGGGCggCCCGUGCGg -3' miRNA: 3'- gCCaUUA---CUCUAUgCUCG--GGGCGCGC- -5' |
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5979 | 5' | -54.8 | NC_001806.1 | + | 147344 | 0.66 | 0.941706 |
Target: 5'- uCGGaGggGGGGUGCGucgccgcccucuuGGCCCCugccgGCGCGa -3' miRNA: 3'- -GCCaUuaCUCUAUGC-------------UCGGGG-----CGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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