Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5994 | 3' | -57.1 | NC_001806.1 | + | 131958 | 0.69 | 0.694156 |
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Target: 5'- -aCGCGUgcGUCCUggcgGCCCUGGAGCg--- -3' miRNA: 3'- ggGCGUA--UAGGG----UGGGACCUCGaguu -5' |
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| 5994 | 3' | -57.1 | NC_001806.1 | + | 23588 | 0.69 | 0.673857 |
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Target: 5'- gCCCGCGgccgCCGCCCUGGAggccuacuGCUCc- -3' miRNA: 3'- -GGGCGUauagGGUGGGACCU--------CGAGuu -5' |
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| 5994 | 3' | -57.1 | NC_001806.1 | + | 24345 | 0.69 | 0.663652 |
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Target: 5'- gCCGCGUGUUCgGCCCgGGGGUcuUCGc -3' miRNA: 3'- gGGCGUAUAGGgUGGGaCCUCG--AGUu -5' |
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| 5994 | 3' | -57.1 | NC_001806.1 | + | 17856 | 0.7 | 0.653421 |
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Target: 5'- cCCCGCGgccCCCAaccaccCCCUGGAgacccuGCUCAGc -3' miRNA: 3'- -GGGCGUauaGGGU------GGGACCU------CGAGUU- -5' |
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| 5994 | 3' | -57.1 | NC_001806.1 | + | 51738 | 0.7 | 0.62266 |
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Target: 5'- gCUGCGg--CCCACCCUGGcGC-CAAa -3' miRNA: 3'- gGGCGUauaGGGUGGGACCuCGaGUU- -5' |
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| 5994 | 3' | -57.1 | NC_001806.1 | + | 22669 | 0.72 | 0.521665 |
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Target: 5'- gCCGCGgggAUCCUcgccGCCCUGGGGCg--- -3' miRNA: 3'- gGGCGUa--UAGGG----UGGGACCUCGaguu -5' |
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| 5994 | 3' | -57.1 | NC_001806.1 | + | 98798 | 0.72 | 0.511866 |
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Target: 5'- aCCCGUAa---CCACCaaGGAGCUCAAg -3' miRNA: 3'- -GGGCGUauagGGUGGgaCCUCGAGUU- -5' |
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| 5994 | 3' | -57.1 | NC_001806.1 | + | 94229 | 0.72 | 0.492503 |
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Target: 5'- cCCCGC-UGUCggUCACCCUGGAGUUg-- -3' miRNA: 3'- -GGGCGuAUAG--GGUGGGACCUCGAguu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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