Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6001 | 5' | -58.1 | NC_001806.1 | + | 146069 | 0.7 | 0.585363 |
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Target: 5'- cGGccAGAGGccAGGUCaGUCCGGGCGggCa -3' miRNA: 3'- aCCa-UCUCC--UCCGG-CAGGCCUGCuaG- -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 27026 | 0.7 | 0.585363 |
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Target: 5'- gGGUGGcGGAGGCCGa--GG-CGGUCa -3' miRNA: 3'- aCCAUCuCCUCCGGCaggCCuGCUAG- -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 4418 | 0.71 | 0.5754 |
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Target: 5'- cGGagAGGGGGGGUgGcCCGGGCGGg- -3' miRNA: 3'- aCCa-UCUCCUCCGgCaGGCCUGCUag -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 151334 | 0.71 | 0.555598 |
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Target: 5'- cGGgcG-GGGGGCUGUCCGGcCaGUCg -3' miRNA: 3'- aCCauCuCCUCCGGCAGGCCuGcUAG- -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 99606 | 0.74 | 0.407655 |
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Target: 5'- cGGUAGAGGGGcGCgCGUCUgGGugGAa- -3' miRNA: 3'- aCCAUCUCCUC-CG-GCAGG-CCugCUag -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 23946 | 0.74 | 0.39923 |
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Target: 5'- gGGgccuGGGGGGGCCG-CCGGAgUGGUCc -3' miRNA: 3'- aCCau--CUCCUCCGGCaGGCCU-GCUAG- -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 74057 | 0.74 | 0.381904 |
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Target: 5'- cGGcUGGAGGAGGugcggacccaggcCCGU-CGGGCGAUCg -3' miRNA: 3'- aCC-AUCUCCUCC-------------GGCAgGCCUGCUAG- -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 10393 | 0.75 | 0.366666 |
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Target: 5'- gGGgcgGGGGGAGGCgGgagCCGGGgGGUCc -3' miRNA: 3'- aCCa--UCUCCUCCGgCa--GGCCUgCUAG- -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 29447 | 0.79 | 0.205935 |
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Target: 5'- aGGccGAGGAGGCCGUCaGGGCGggCa -3' miRNA: 3'- aCCauCUCCUCCGGCAGgCCUGCuaG- -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 21380 | 1.08 | 0.00209 |
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Target: 5'- aUGGUAGAGGAGGCCGUCCGGACGAUCc -3' miRNA: 3'- -ACCAUCUCCUCCGGCAGGCCUGCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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