Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 607 | 3' | -64.1 | AC_000017.1 | + | 5362 | 0.7 | 0.118409 |
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Target: 5'- uCCgGGCU-GCGCGCuGGCCagGGuGCGCu -3' miRNA: 3'- -GGaCCGGuCGCGCG-UCGGg-CCuCGCG- -5' |
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| 607 | 3' | -64.1 | AC_000017.1 | + | 10824 | 0.7 | 0.1149 |
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Target: 5'- aCUGGCC-GCGCGCGGCguaagcgguuaggCUGGAaaGCg -3' miRNA: 3'- gGACCGGuCGCGCGUCG-------------GGCCUcgCG- -5' |
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| 607 | 3' | -64.1 | AC_000017.1 | + | 16435 | 0.71 | 0.087432 |
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Target: 5'- --cGGCCuGCGCGU-GCCCGu-GCGCa -3' miRNA: 3'- ggaCCGGuCGCGCGuCGGGCcuCGCG- -5' |
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| 607 | 3' | -64.1 | AC_000017.1 | + | 16067 | 0.71 | 0.085033 |
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Target: 5'- --cGGCCGcguuuGUGCGC-GCCCcaGGGGCGCg -3' miRNA: 3'- ggaCCGGU-----CGCGCGuCGGG--CCUCGCG- -5' |
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| 607 | 3' | -64.1 | AC_000017.1 | + | 5491 | 0.72 | 0.076051 |
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Target: 5'- -aUGGCCcuuGGCGCGCAGCUUgcccuuggaGGAgGCGCc -3' miRNA: 3'- ggACCGG---UCGCGCGUCGGG---------CCU-CGCG- -5' |
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| 607 | 3' | -64.1 | AC_000017.1 | + | 11245 | 0.73 | 0.066098 |
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Target: 5'- --gGGCCuG-GCGCGGCuaGGAGCGCc -3' miRNA: 3'- ggaCCGGuCgCGCGUCGggCCUCGCG- -5' |
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| 607 | 3' | -64.1 | AC_000017.1 | + | 22795 | 0.77 | 0.031032 |
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Target: 5'- gCUGGCCAGCGUGCAucaCCCGGcgcugauaguguuccAGUGCu -3' miRNA: 3'- gGACCGGUCGCGCGUc--GGGCC---------------UCGCG- -5' |
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| 607 | 3' | -64.1 | AC_000017.1 | + | 5485 | 0.79 | 0.022367 |
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Target: 5'- aCCUGGCCGaCGCGCAGggcgaagaCCGGcAGCGCu -3' miRNA: 3'- -GGACCGGUcGCGCGUCg-------GGCC-UCGCG- -5' |
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| 607 | 3' | -64.1 | AC_000017.1 | + | 25543 | 0.8 | 0.017761 |
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Target: 5'- ---cGCCAgGUGCGCGGCCaCGGAGCGCu -3' miRNA: 3'- ggacCGGU-CGCGCGUCGG-GCCUCGCG- -5' |
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| 607 | 3' | -64.1 | AC_000017.1 | + | 5419 | 1.12 | 0.000043 |
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Target: 5'- cCCUGGCCAGCGCGCAGCCCGGAGCGCa -3' miRNA: 3'- -GGACCGGUCGCGCGUCGGGCCUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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