Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 619 | 5' | -58.9 | AC_000017.1 | + | 18329 | 0.69 | 0.290264 |
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Target: 5'- cCCGCCcuCCCGuagaGGAGCCuCCAC-CGg -3' miRNA: 3'- -GGUGGuuGGGC----CCUCGGuGGUGaGCg -5' |
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| 619 | 5' | -58.9 | AC_000017.1 | + | 18965 | 0.7 | 0.23066 |
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Target: 5'- gCACCAGCCCGGG-GCUcaGgUACUCcgagGCg -3' miRNA: 3'- gGUGGUUGGGCCCuCGG--UgGUGAG----CG- -5' |
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| 619 | 5' | -58.9 | AC_000017.1 | + | 20455 | 1.12 | 0.000167 |
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Target: 5'- uCCACCAACCCGGGAGCCACCACUCGCu -3' miRNA: 3'- -GGUGGUUGGGCCCUCGGUGGUGAGCG- -5' |
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| 619 | 5' | -58.9 | AC_000017.1 | + | 23317 | 0.67 | 0.396518 |
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Target: 5'- aCCACgAGCCCacGGGcugcgcguuguGGCugCACCGCUgCGCu -3' miRNA: 3'- -GGUGgUUGGG--CCC-----------UCG--GUGGUGA-GCG- -5' |
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| 619 | 5' | -58.9 | AC_000017.1 | + | 24035 | 0.7 | 0.255119 |
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Target: 5'- uCCACCAACCUGGaGGacgugucguccccGUCGCCG-UCGCc -3' miRNA: 3'- -GGUGGUUGGGCC-CU-------------CGGUGGUgAGCG- -5' |
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| 619 | 5' | -58.9 | AC_000017.1 | + | 26016 | 0.69 | 0.283085 |
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Target: 5'- cCCGCCcGCCCaaugcGGAGCuUACCGCcUGCg -3' miRNA: 3'- -GGUGGuUGGGc----CCUCG-GUGGUGaGCG- -5' |
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| 619 | 5' | -58.9 | AC_000017.1 | + | 26523 | 0.66 | 0.437734 |
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Target: 5'- aCCACUggAACCaGGGccgguaaguccaagcAGCCGCCGC-CGUu -3' miRNA: 3'- -GGUGG--UUGGgCCC---------------UCGGUGGUGaGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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