Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
619 | 5' | -58.9 | AC_000017.1 | + | 8986 | 0.67 | 0.378628 |
Target: 5'- gCCGCCA--CCGuGGAGCgaGCCGgaCGCg -3' miRNA: 3'- -GGUGGUugGGC-CCUCGg-UGGUgaGCG- -5' |
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619 | 5' | -58.9 | AC_000017.1 | + | 14997 | 0.67 | 0.396518 |
Target: 5'- -aGCCcuCCUGGaaGGGUUGCCGCUUGCg -3' miRNA: 3'- ggUGGuuGGGCC--CUCGGUGGUGAGCG- -5' |
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619 | 5' | -58.9 | AC_000017.1 | + | 23317 | 0.67 | 0.396518 |
Target: 5'- aCCACgAGCCCacGGGcugcgcguuguGGCugCACCGCUgCGCu -3' miRNA: 3'- -GGUGgUUGGG--CCC-----------UCG--GUGGUGA-GCG- -5' |
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619 | 5' | -58.9 | AC_000017.1 | + | 17521 | 0.66 | 0.414944 |
Target: 5'- aCCACUggAACCCGcc-GCCGCCG-UCGCc -3' miRNA: 3'- -GGUGG--UUGGGCccuCGGUGGUgAGCG- -5' |
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619 | 5' | -58.9 | AC_000017.1 | + | 26523 | 0.66 | 0.437734 |
Target: 5'- aCCACUggAACCaGGGccgguaaguccaagcAGCCGCCGC-CGUu -3' miRNA: 3'- -GGUGG--UUGGgCCC---------------UCGGUGGUGaGCG- -5' |
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619 | 5' | -58.9 | AC_000017.1 | + | 17341 | 0.66 | 0.443543 |
Target: 5'- gCCGCCuGCaCCGcGGcaucuGCCACCGC-CGa -3' miRNA: 3'- -GGUGGuUG-GGC-CCu----CGGUGGUGaGCg -5' |
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619 | 5' | -58.9 | AC_000017.1 | + | 6647 | 0.66 | 0.45332 |
Target: 5'- gCUGCCAugCgCGGGcGGCaagCGCgCGCUCGUa -3' miRNA: 3'- -GGUGGUugG-GCCC-UCG---GUG-GUGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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