Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 619 | 5' | -58.9 | AC_000017.1 | + | 4176 | 0.71 | 0.196918 |
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Target: 5'- aCCGCUGGuCCCGGGc-CUACCGCgcgCGCa -3' miRNA: 3'- -GGUGGUU-GGGCCCucGGUGGUGa--GCG- -5' |
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| 619 | 5' | -58.9 | AC_000017.1 | + | 15726 | 0.71 | 0.196918 |
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Target: 5'- cUCACaGAUCaCGGGAcGCUACCGCUgCGCa -3' miRNA: 3'- -GGUGgUUGG-GCCCU-CGGUGGUGA-GCG- -5' |
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| 619 | 5' | -58.9 | AC_000017.1 | + | 16269 | 0.73 | 0.163073 |
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Target: 5'- gCACCGGCCgaCGGGcGGCCaugcgaGCCGCUCGa -3' miRNA: 3'- gGUGGUUGG--GCCC-UCGG------UGGUGAGCg -5' |
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| 619 | 5' | -58.9 | AC_000017.1 | + | 11554 | 0.73 | 0.142197 |
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Target: 5'- uUACCAGgUCGGcGGCCGCCACgugugCGCg -3' miRNA: 3'- gGUGGUUgGGCCcUCGGUGGUGa----GCG- -5' |
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| 619 | 5' | -58.9 | AC_000017.1 | + | 16781 | 0.74 | 0.127287 |
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Target: 5'- aCCACCGuagucuuuacGCCCGGuGAGCgCuCCACcCGCa -3' miRNA: 3'- -GGUGGU----------UGGGCC-CUCG-GuGGUGaGCG- -5' |
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| 619 | 5' | -58.9 | AC_000017.1 | + | 16209 | 0.78 | 0.062601 |
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Target: 5'- gCCGCCGACCCGGcacuGCCGCCcaACgCGCg -3' miRNA: 3'- -GGUGGUUGGGCCcu--CGGUGG--UGaGCG- -5' |
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| 619 | 5' | -58.9 | AC_000017.1 | + | 20455 | 1.12 | 0.000167 |
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Target: 5'- uCCACCAACCCGGGAGCCACCACUCGCu -3' miRNA: 3'- -GGUGGUUGGGCCCUCGGUGGUGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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