Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6197 | 5' | -51.9 | NC_001844.1 | + | 79970 | 0.66 | 0.98527 |
Target: 5'- gGCGcGGAUU--GAGCGGGu--GGCGCg -3' miRNA: 3'- -CGUaCCUGGuuUUCGCCUcuuCCGCG- -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 118965 | 0.66 | 0.985091 |
Target: 5'- -gAUGGGgUggGGGCGGgAGGugaaaauGGGCGUg -3' miRNA: 3'- cgUACCUgGuuUUCGCC-UCU-------UCCGCG- -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 100395 | 0.66 | 0.983403 |
Target: 5'- uCAUGGgggcGCCAAAGcCGGAGAacucGGGCa- -3' miRNA: 3'- cGUACC----UGGUUUUcGCCUCU----UCCGcg -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 116741 | 0.66 | 0.981362 |
Target: 5'- --cUGGAUCGuacaccccaaAGAGCGGAcgcucagaGAAGGgGCa -3' miRNA: 3'- cguACCUGGU----------UUUCGCCU--------CUUCCgCG- -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 826 | 0.66 | 0.979139 |
Target: 5'- uCGUGGuuACCAGAAGCacgacGGuGAcacccuGGCGCg -3' miRNA: 3'- cGUACC--UGGUUUUCG-----CCuCUu-----CCGCG- -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 139626 | 0.67 | 0.974113 |
Target: 5'- cGCGUGcGuuauuucCCAgGAAGCGGAaauGGCGCa -3' miRNA: 3'- -CGUAC-Cu------GGU-UUUCGCCUcuuCCGCG- -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 126101 | 0.67 | 0.971293 |
Target: 5'- gGCAUGgGACCGGuuuuGCGGGGuucugcauagcaGAGGuCGUc -3' miRNA: 3'- -CGUAC-CUGGUUuu--CGCCUC------------UUCC-GCG- -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 1149 | 0.67 | 0.968259 |
Target: 5'- -gGUGGGCaCAcAGAGaUGGAuaccgaGGAGGCGCg -3' miRNA: 3'- cgUACCUG-GU-UUUC-GCCU------CUUCCGCG- -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 61662 | 0.67 | 0.968259 |
Target: 5'- -uGUGGAuCCAGuAGGCGGcGGGcAGGUGUa -3' miRNA: 3'- cgUACCU-GGUU-UUCGCC-UCU-UCCGCG- -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 144224 | 0.67 | 0.965005 |
Target: 5'- ---aGGACCGcgcGGCuGAGguGGCGCu -3' miRNA: 3'- cguaCCUGGUuu-UCGcCUCuuCCGCG- -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 80873 | 0.68 | 0.961523 |
Target: 5'- gGCAUcagcuGACCAAAgauagGGCGGGGAccuugggguccuAGGCGg -3' miRNA: 3'- -CGUAc----CUGGUUU-----UCGCCUCU------------UCCGCg -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 120047 | 0.68 | 0.961523 |
Target: 5'- gGCGUuuuguucGGCCAaaacGAGGCGGGGGucgcgauuGGCGCu -3' miRNA: 3'- -CGUAc------CUGGU----UUUCGCCUCUu-------CCGCG- -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 105960 | 0.68 | 0.957808 |
Target: 5'- aGCAUGGACguGuuuGGGCG-AGGAcGCGCu -3' miRNA: 3'- -CGUACCUGguU---UUCGCcUCUUcCGCG- -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 121869 | 0.68 | 0.957808 |
Target: 5'- -aGUGGGCgCAAAAaaGGAGGcGGGUGCu -3' miRNA: 3'- cgUACCUG-GUUUUcgCCUCU-UCCGCG- -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 65429 | 0.68 | 0.957808 |
Target: 5'- -aGUGGAgCGuAGAGCGGGGc--GCGCg -3' miRNA: 3'- cgUACCUgGU-UUUCGCCUCuucCGCG- -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 113177 | 0.68 | 0.945216 |
Target: 5'- gGUAUcGGACgAGGAGgaCGGAGAAgacuGGUGCa -3' miRNA: 3'- -CGUA-CCUGgUUUUC--GCCUCUU----CCGCG- -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 116302 | 0.69 | 0.93558 |
Target: 5'- cCAUGGcCCGGGucGGacgccccaccacCGGGGAGGGUGCg -3' miRNA: 3'- cGUACCuGGUUU--UC------------GCCUCUUCCGCG- -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 16762 | 0.7 | 0.907053 |
Target: 5'- aGCcUGGGCU---GGCaGAGuGGGCGCg -3' miRNA: 3'- -CGuACCUGGuuuUCGcCUCuUCCGCG- -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 138759 | 0.7 | 0.907053 |
Target: 5'- ---cGGugUgcguGUGGGGggGGCGCa -3' miRNA: 3'- cguaCCugGuuuuCGCCUCuuCCGCG- -5' |
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6197 | 5' | -51.9 | NC_001844.1 | + | 5151 | 0.7 | 0.893881 |
Target: 5'- ---cGGACCGucuGGCGGGu--GGCGCu -3' miRNA: 3'- cguaCCUGGUuu-UCGCCUcuuCCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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