Results 1 - 20 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6203 | 5' | -56.4 | NC_001844.1 | + | 102013 | 0.66 | 0.896119 |
Target: 5'- uUCGGAggagguaGACGGCGUUAuuuucuccUCUGAGCCCUc -3' miRNA: 3'- -AGCCUa------UUGUCGCGGU--------GGGUUCGGGG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 113230 | 0.66 | 0.889462 |
Target: 5'- -gGGGUGagcccgGCAGCGCaguugAUCCGAcgcGCCCCc -3' miRNA: 3'- agCCUAU------UGUCGCGg----UGGGUU---CGGGG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 114976 | 0.66 | 0.868153 |
Target: 5'- gCGGGgggccGACAaccugcucccGgGCCACCCAGacccaggcucGCCCCu -3' miRNA: 3'- aGCCUa----UUGU----------CgCGGUGGGUU----------CGGGG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 115265 | 0.66 | 0.868153 |
Target: 5'- gCGGA-GGgAGC-CCggGCCCGGGCCUCg -3' miRNA: 3'- aGCCUaUUgUCGcGG--UGGGUUCGGGG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 116481 | 0.67 | 0.860619 |
Target: 5'- cCGGGgccucuaAGCGcaagcGCuGCCACCCAAGaCCCa -3' miRNA: 3'- aGCCUa------UUGU-----CG-CGGUGGGUUCgGGG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 101000 | 0.67 | 0.860619 |
Target: 5'- gCGGAUuuGCGGCGaaguguguaaCugCCAcGCCCUa -3' miRNA: 3'- aGCCUAu-UGUCGCg---------GugGGUuCGGGG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 113311 | 0.67 | 0.860619 |
Target: 5'- cUGGA--GCuGUGCCuuCCCGAGUCCg -3' miRNA: 3'- aGCCUauUGuCGCGGu-GGGUUCGGGg -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 144547 | 0.67 | 0.85288 |
Target: 5'- cCGGGc-GCAGCcauGCC-CCaAGGCCCCa -3' miRNA: 3'- aGCCUauUGUCG---CGGuGGgUUCGGGG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 78850 | 0.67 | 0.836807 |
Target: 5'- gCGGggGGUAGgGUUACgUAAGCCCCa -3' miRNA: 3'- aGCCuaUUGUCgCGGUGgGUUCGGGG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 120358 | 0.67 | 0.836807 |
Target: 5'- gCGGAgcGCAGaaCGCU-CCCG-GCCCCc -3' miRNA: 3'- aGCCUauUGUC--GCGGuGGGUuCGGGG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 144296 | 0.67 | 0.835984 |
Target: 5'- uUCGGGUAGaGGCGCgCaaacgcgGCCUcGGCCCUc -3' miRNA: 3'- -AGCCUAUUgUCGCG-G-------UGGGuUCGGGG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 138692 | 0.67 | 0.834331 |
Target: 5'- gUUGGGUAgguugcuagcuggcACAGUGCCAUgCGcGCuCCCg -3' miRNA: 3'- -AGCCUAU--------------UGUCGCGGUGgGUuCG-GGG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 116381 | 0.67 | 0.819991 |
Target: 5'- gUUGGAcaugAACuGCuCCuCUCAGGCCCCa -3' miRNA: 3'- -AGCCUa---UUGuCGcGGuGGGUUCGGGG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 142214 | 0.68 | 0.811324 |
Target: 5'- gCGGucGUAGCGGCggcucaugGCCACCgAGGCUgCCg -3' miRNA: 3'- aGCC--UAUUGUCG--------CGGUGGgUUCGG-GG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 13081 | 0.68 | 0.811324 |
Target: 5'- aCcGAUGGgGGCGaCCGCUgcAGCCCCa -3' miRNA: 3'- aGcCUAUUgUCGC-GGUGGguUCGGGG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 136648 | 0.68 | 0.802494 |
Target: 5'- cCGGu---CAGUG-CGCCUAGGCUCCa -3' miRNA: 3'- aGCCuauuGUCGCgGUGGGUUCGGGG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 15937 | 0.68 | 0.802494 |
Target: 5'- cCGGGUGgugaAUAGCGCgCACCCAugacugguaAGCgCUg -3' miRNA: 3'- aGCCUAU----UGUCGCG-GUGGGU---------UCGgGG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 60746 | 0.68 | 0.802494 |
Target: 5'- gCGGuuaucuaugauuGUAcCGGCGCCACUCAccGCCUCg -3' miRNA: 3'- aGCC------------UAUuGUCGCGGUGGGUu-CGGGG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 5420 | 0.68 | 0.793509 |
Target: 5'- gUGGugaguuuuGCAGCGUCGCCUAAaaaCCCCa -3' miRNA: 3'- aGCCuau-----UGUCGCGGUGGGUUc--GGGG- -5' |
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6203 | 5' | -56.4 | NC_001844.1 | + | 141775 | 0.68 | 0.793509 |
Target: 5'- cCGGAUGgugugaGCAGCgGCuCGCgcuggcagguuCCGAGCCCUg -3' miRNA: 3'- aGCCUAU------UGUCG-CG-GUG-----------GGUUCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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