Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6349 | 5' | -56.6 | NC_001847.1 | + | 116960 | 0.66 | 0.865856 |
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Target: 5'- gCGGUGcucCGGCUgcccugcgaCGC-CGACCGCCUc -3' miRNA: 3'- aGUCACa--GCUGA---------GCGuGCUGGCGGAc -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 120121 | 0.68 | 0.75344 |
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Target: 5'- cCGGcGgccgCGGCUCGCGCGGCC-CCa- -3' miRNA: 3'- aGUCaCa---GCUGAGCGUGCUGGcGGac -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 128441 | 0.67 | 0.834315 |
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Target: 5'- aCAGUGcgcUCGGCccugCGCGCGcgcccggccgGCCGCCg- -3' miRNA: 3'- aGUCAC---AGCUGa---GCGUGC----------UGGCGGac -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 130245 | 0.66 | 0.873233 |
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Target: 5'- gUCGGUGUCG-UUCGCAgCGGCgCGggggcCCUGc -3' miRNA: 3'- -AGUCACAGCuGAGCGU-GCUG-GC-----GGAC- -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 131009 | 1.08 | 0.002782 |
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Target: 5'- cUCAGUGUCGACUCGCACGACCGCCUGu -3' miRNA: 3'- -AGUCACAGCUGAGCGUGCUGGCGGAC- -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 131805 | 0.71 | 0.631317 |
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Target: 5'- gCAGcGUCGGCauaacgcaccccUGCGCGGCCGCCUa -3' miRNA: 3'- aGUCaCAGCUGa-----------GCGUGCUGGCGGAc -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 134504 | 0.69 | 0.714343 |
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Target: 5'- gUAGUGUgGGC-CGUGCGGCCGCg-- -3' miRNA: 3'- aGUCACAgCUGaGCGUGCUGGCGgac -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 134644 | 0.68 | 0.79985 |
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Target: 5'- aCAGggggGUUGg--CGCGCGGCCGCCc- -3' miRNA: 3'- aGUCa---CAGCugaGCGUGCUGGCGGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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