Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6349 | 5' | -56.6 | NC_001847.1 | + | 58237 | 0.7 | 0.653778 |
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Target: 5'- gCGGcagCGGCggCGCGCGACCGCCc- -3' miRNA: 3'- aGUCacaGCUGa-GCGUGCUGGCGGac -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 102541 | 0.7 | 0.674127 |
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Target: 5'- cUCcGUGUCGGC-CGCgACGGCgGCCg- -3' miRNA: 3'- -AGuCACAGCUGaGCG-UGCUGgCGGac -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 134504 | 0.69 | 0.714343 |
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Target: 5'- gUAGUGUgGGC-CGUGCGGCCGCg-- -3' miRNA: 3'- aGUCACAgCUGaGCGUGCUGGCGgac -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 73191 | 0.69 | 0.743803 |
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Target: 5'- gCGGaGUCGGCcCGCGCGggccgcGCCGCCg- -3' miRNA: 3'- aGUCaCAGCUGaGCGUGC------UGGCGGac -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 120121 | 0.68 | 0.75344 |
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Target: 5'- cCGGcGgccgCGGCUCGCGCGGCC-CCa- -3' miRNA: 3'- aGUCaCa---GCUGAGCGUGCUGGcGGac -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 47867 | 0.68 | 0.762972 |
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Target: 5'- gCGGgccGUCGGCgCGCACGuCCGCgaGa -3' miRNA: 3'- aGUCa--CAGCUGaGCGUGCuGGCGgaC- -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 65504 | 0.68 | 0.762972 |
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Target: 5'- gCGGUGUggggCGGCgcgCGCaguGCGGCCGCCc- -3' miRNA: 3'- aGUCACA----GCUGa--GCG---UGCUGGCGGac -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 80539 | 0.66 | 0.887337 |
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Target: 5'- gUCGGcaUCGGCgcgggCGCGCG-CCGCCa- -3' miRNA: 3'- -AGUCacAGCUGa----GCGUGCuGGCGGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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