Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6349 | 5' | -56.6 | NC_001847.1 | + | 57559 | 0.66 | 0.872505 |
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Target: 5'- -gGGUGccgccgugcgagcUCGGCcCGCGCGGCCGCg-- -3' miRNA: 3'- agUCAC-------------AGCUGaGCGUGCUGGCGgac -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 130245 | 0.66 | 0.873233 |
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Target: 5'- gUCGGUGUCG-UUCGCAgCGGCgCGggggcCCUGc -3' miRNA: 3'- -AGUCACAGCuGAGCGU-GCUG-GC-----GGAC- -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 106293 | 0.66 | 0.873233 |
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Target: 5'- cCGGcGUCG-CgCGCGCGGCCGCg-- -3' miRNA: 3'- aGUCaCAGCuGaGCGUGCUGGCGgac -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 27432 | 0.66 | 0.873233 |
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Target: 5'- gUCGGUGUCG-UUCGCAgCGGCgCGggggcCCUGc -3' miRNA: 3'- -AGUCACAGCuGAGCGU-GCUG-GC-----GGAC- -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 79377 | 0.66 | 0.880395 |
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Target: 5'- gCuGUG-CGGCagCGCGCGGCuCGCCg- -3' miRNA: 3'- aGuCACaGCUGa-GCGUGCUG-GCGGac -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 80539 | 0.66 | 0.887337 |
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Target: 5'- gUCGGcaUCGGCgcgggCGCGCG-CCGCCa- -3' miRNA: 3'- -AGUCacAGCUGa----GCGUGCuGGCGGac -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 52500 | 0.66 | 0.887337 |
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Target: 5'- -uGGUGgagcUGACggagCGCGCGGCCGCg-- -3' miRNA: 3'- agUCACa---GCUGa---GCGUGCUGGCGgac -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 96864 | 0.66 | 0.887337 |
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Target: 5'- gCGGUGggCGGCggucCGCGCuggggaggGACCGCCg- -3' miRNA: 3'- aGUCACa-GCUGa---GCGUG--------CUGGCGGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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