Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6349 | 5' | -56.6 | NC_001847.1 | + | 19502 | 0.71 | 0.633361 |
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Target: 5'- gUCGG-GUCGugUCGCGgGAUCGCgCUu -3' miRNA: 3'- -AGUCaCAGCugAGCGUgCUGGCG-GAc -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 131805 | 0.71 | 0.631317 |
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Target: 5'- gCAGcGUCGGCauaacgcaccccUGCGCGGCCGCCUa -3' miRNA: 3'- aGUCaCAGCUGa-----------GCGUGCUGGCGGAc -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 55741 | 0.71 | 0.602746 |
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Target: 5'- aCGGUGgucCGGCUCGCgcccgcGCGGCCGugcCCUGa -3' miRNA: 3'- aGUCACa--GCUGAGCG------UGCUGGC---GGAC- -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 19636 | 0.71 | 0.582432 |
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Target: 5'- cCGGcGUCGg--CGCGCGGCCGCUUGc -3' miRNA: 3'- aGUCaCAGCugaGCGUGCUGGCGGAC- -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 32895 | 0.72 | 0.542282 |
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Target: 5'- aCGGUG-CGcGCcgCGCGCGAgCGCCUGg -3' miRNA: 3'- aGUCACaGC-UGa-GCGUGCUgGCGGAC- -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 66349 | 0.75 | 0.385804 |
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Target: 5'- ----cGUCGgcccGCUCGCGCGGCCGCCg- -3' miRNA: 3'- agucaCAGC----UGAGCGUGCUGGCGGac -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 101560 | 0.77 | 0.315902 |
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Target: 5'- gCAGgacgucGUCGGCaccgCGCACGGCCGCCUc -3' miRNA: 3'- aGUCa-----CAGCUGa---GCGUGCUGGCGGAc -5' |
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| 6349 | 5' | -56.6 | NC_001847.1 | + | 131009 | 1.08 | 0.002782 |
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Target: 5'- cUCAGUGUCGACUCGCACGACCGCCUGu -3' miRNA: 3'- -AGUCACAGCUGAGCGUGCUGGCGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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