Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6374 | 5' | -48.4 | NC_001847.1 | + | 74461 | 0.66 | 0.998511 |
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Target: 5'- gGCGCUGcAGUACGAgcGGGGGcugggcgUGUugaugcagcagccgcGCCUg -3' miRNA: 3'- -CGCGACuUCAUGCU--UCUCU-------ACA---------------UGGA- -5' |
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| 6374 | 5' | -48.4 | NC_001847.1 | + | 101779 | 0.66 | 0.998674 |
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Target: 5'- gGCGCagacugcugagggUGAGGUGCGAGGGGccc-ACCg -3' miRNA: 3'- -CGCG-------------ACUUCAUGCUUCUCuacaUGGa -5' |
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| 6374 | 5' | -48.4 | NC_001847.1 | + | 3936 | 0.66 | 0.9987 |
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Target: 5'- cGCGCUGgcGccgcgGCGggGGGGgc-GCCg -3' miRNA: 3'- -CGCGACuuCa----UGCuuCUCUacaUGGa -5' |
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| 6374 | 5' | -48.4 | NC_001847.1 | + | 106749 | 0.66 | 0.9987 |
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Target: 5'- cGCGCUGgcGccgcgGCGggGGGGgc-GCCg -3' miRNA: 3'- -CGCGACuuCa----UGCuuCUCUacaUGGa -5' |
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| 6374 | 5' | -48.4 | NC_001847.1 | + | 54662 | 0.66 | 0.998935 |
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Target: 5'- cGCGCUGcGGaACGggGAGcgcgGCCc -3' miRNA: 3'- -CGCGACuUCaUGCuuCUCuacaUGGa -5' |
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| 6374 | 5' | -48.4 | NC_001847.1 | + | 34719 | 0.66 | 0.999133 |
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Target: 5'- cGCGCUGGAGcGCGucGAGgcGaACUc -3' miRNA: 3'- -CGCGACUUCaUGCuuCUCuaCaUGGa -5' |
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| 6374 | 5' | -48.4 | NC_001847.1 | + | 26089 | 0.66 | 0.999133 |
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Target: 5'- aUGCUGggG-GCGGAGGGAgcaGUGgCg -3' miRNA: 3'- cGCGACuuCaUGCUUCUCUa--CAUgGa -5' |
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| 6374 | 5' | -48.4 | NC_001847.1 | + | 128902 | 0.66 | 0.999133 |
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Target: 5'- aUGCUGggG-GCGGAGGGAgcaGUGgCg -3' miRNA: 3'- cGCGACuuCaUGCUUCUCUa--CAUgGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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