Results 1 - 20 of 98 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6447 | 3' | -56.3 | NC_001847.1 | + | 5469 | 0.66 | 0.857463 |
Target: 5'- --gCCGCUCuCUGCgg-CGGCGGCa- -3' miRNA: 3'- uuaGGCGGGcGAUGaaaGUCGCCGac -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 68914 | 0.66 | 0.857463 |
Target: 5'- --gCCGCCgCGCUGaggUUCGGCgcggcggccgcgGGCUGc -3' miRNA: 3'- uuaGGCGG-GCGAUga-AAGUCG------------CCGAC- -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 86531 | 0.66 | 0.857463 |
Target: 5'- ---gCGCCCGCgUGCUggCGgcGCGGCUu -3' miRNA: 3'- uuagGCGGGCG-AUGAaaGU--CGCCGAc -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 1232 | 0.66 | 0.850258 |
Target: 5'- --gCCGCCCGCaggccagguacaccgGCcg-CAGCGGCg- -3' miRNA: 3'- uuaGGCGGGCGa--------------UGaaaGUCGCCGac -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 104045 | 0.66 | 0.850258 |
Target: 5'- --gCCGCCCGCaggccagguacaccgGCcg-CAGCGGCg- -3' miRNA: 3'- uuaGGCGGGCGa--------------UGaaaGUCGCCGac -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 123829 | 0.66 | 0.849446 |
Target: 5'- --gCCGCCCGCcgGCc-UCGGCgGGCg- -3' miRNA: 3'- uuaGGCGGGCGa-UGaaAGUCG-CCGac -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 105812 | 0.66 | 0.849446 |
Target: 5'- --gCCGCCCGCgcACUcgCGcucGCGGCUc -3' miRNA: 3'- uuaGGCGGGCGa-UGAaaGU---CGCCGAc -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 97871 | 0.66 | 0.849446 |
Target: 5'- --gCgGCCCGCgucccagUCGGCGGCg- -3' miRNA: 3'- uuaGgCGGGCGaugaa--AGUCGCCGac -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 79141 | 0.66 | 0.849446 |
Target: 5'- -uUgCGCCCGCUGCg--CAGguacuCGGCUu -3' miRNA: 3'- uuAgGCGGGCGAUGaaaGUC-----GCCGAc -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 92655 | 0.66 | 0.849446 |
Target: 5'- -uUUCGCCCGCaGCgcUUCGGCGcGCc- -3' miRNA: 3'- uuAGGCGGGCGaUGa-AAGUCGC-CGac -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 77339 | 0.66 | 0.849446 |
Target: 5'- --aCUGCgCGCUGCUg--GGCGGCg- -3' miRNA: 3'- uuaGGCGgGCGAUGAaagUCGCCGac -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 82963 | 0.66 | 0.849446 |
Target: 5'- --gCCGCCUGCgauCUgu-GGUGGCUGc -3' miRNA: 3'- uuaGGCGGGCGau-GAaagUCGCCGAC- -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 14653 | 0.66 | 0.842055 |
Target: 5'- --gCCGCUCGCUGCgagcggggcgcgugUCGGCGuGCa- -3' miRNA: 3'- uuaGGCGGGCGAUGaa------------AGUCGC-CGac -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 60130 | 0.66 | 0.841223 |
Target: 5'- -uUCUGCCCGCUggccccccGCccgcCGGCGGaCUGa -3' miRNA: 3'- uuAGGCGGGCGA--------UGaaa-GUCGCC-GAC- -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 46100 | 0.66 | 0.841223 |
Target: 5'- --cCCgGCCCGCUuccg-CGGgGGCUGg -3' miRNA: 3'- uuaGG-CGGGCGAugaaaGUCgCCGAC- -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 5951 | 0.66 | 0.841223 |
Target: 5'- --cCCGCCCGCUGCUUcUAGCc---- -3' miRNA: 3'- uuaGGCGGGCGAUGAAaGUCGccgac -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 125028 | 0.66 | 0.841223 |
Target: 5'- --cCCGCUCGCcGCgaa-GGCGGCUu -3' miRNA: 3'- uuaGGCGGGCGaUGaaagUCGCCGAc -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 108709 | 0.66 | 0.832801 |
Target: 5'- --gCCuaCCGCUGCcccgUCucGCGGCUGg -3' miRNA: 3'- uuaGGcgGGCGAUGaa--AGu-CGCCGAC- -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 37581 | 0.66 | 0.832801 |
Target: 5'- --gCUGgCCGCUGCggcugCGGCGGCc- -3' miRNA: 3'- uuaGGCgGGCGAUGaaa--GUCGCCGac -5' |
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6447 | 3' | -56.3 | NC_001847.1 | + | 11568 | 0.66 | 0.832801 |
Target: 5'- cGGUCCGgcCCCGCU-Cg--CGGCGGCc- -3' miRNA: 3'- -UUAGGC--GGGCGAuGaaaGUCGCCGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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