Results 1 - 20 of 79 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6456 | 3' | -54.2 | NC_001847.1 | + | 39386 | 0.66 | 0.955192 |
Target: 5'- --cGCCGUcGGGGAGccggcGCCGGccGGGcUCCu -3' miRNA: 3'- guaUGGCGaUUCCUC-----UGGCC--UCC-AGG- -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 3971 | 0.66 | 0.955192 |
Target: 5'- --gGCgGC-GAGGGcGCCGGGGG-CCg -3' miRNA: 3'- guaUGgCGaUUCCUcUGGCCUCCaGG- -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 12946 | 0.66 | 0.955192 |
Target: 5'- uCGUGCgG-UGGGGcggcGGGCCGGGGGUggcCCg -3' miRNA: 3'- -GUAUGgCgAUUCC----UCUGGCCUCCA---GG- -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 29372 | 0.66 | 0.955192 |
Target: 5'- --gACCGCgc-GGcGGCCGGAGcugCCg -3' miRNA: 3'- guaUGGCGauuCCuCUGGCCUCca-GG- -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 18011 | 0.66 | 0.954799 |
Target: 5'- --gGCUGUUGggcguGGGGGgcguugcGCCGGuGGUCCc -3' miRNA: 3'- guaUGGCGAU-----UCCUC-------UGGCCuCCAGG- -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 32958 | 0.66 | 0.951154 |
Target: 5'- -----gGCgGAGGAGGCCGGGGaGgCCa -3' miRNA: 3'- guauggCGaUUCCUCUGGCCUC-CaGG- -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 59038 | 0.66 | 0.951154 |
Target: 5'- gGUGCgGCggcgGAGGAGAgUGGuGGUg- -3' miRNA: 3'- gUAUGgCGa---UUCCUCUgGCCuCCAgg -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 134098 | 0.66 | 0.946879 |
Target: 5'- --cGCCGCUugcucuGGcGAgCaGAGGUCCa -3' miRNA: 3'- guaUGGCGAuu----CCuCUgGcCUCCAGG- -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 110013 | 0.66 | 0.946879 |
Target: 5'- --gGCCGUgGAGGcGAUcaccaCGGAGGUCg -3' miRNA: 3'- guaUGGCGaUUCCuCUG-----GCCUCCAGg -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 100741 | 0.66 | 0.946879 |
Target: 5'- --cGCCGagcucucGGGGCCGG-GGUCCa -3' miRNA: 3'- guaUGGCgauuc--CUCUGGCCuCCAGG- -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 54705 | 0.66 | 0.942364 |
Target: 5'- ---cCCGCgggGAGGAcGACgCGcGGGUCCc -3' miRNA: 3'- guauGGCGa--UUCCU-CUG-GCcUCCAGG- -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 29646 | 0.66 | 0.942364 |
Target: 5'- --cGCCGC--GGGcGGAcCCGGAggacgGGUCCg -3' miRNA: 3'- guaUGGCGauUCC-UCU-GGCCU-----CCAGG- -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 106437 | 0.66 | 0.942364 |
Target: 5'- --cGCCGCgcacaAAGGGGucCCGGuAGG-CCa -3' miRNA: 3'- guaUGGCGa----UUCCUCu-GGCC-UCCaGG- -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 35197 | 0.66 | 0.942364 |
Target: 5'- gAUugUGCgaaguGGGAugGACUGGgcgGGGUCCg -3' miRNA: 3'- gUAugGCGau---UCCU--CUGGCC---UCCAGG- -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 132459 | 0.66 | 0.942364 |
Target: 5'- --cGCCGC--GGGcGGAcCCGGAggacgGGUCCg -3' miRNA: 3'- guaUGGCGauUCC-UCU-GGCCU-----CCAGG- -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 69290 | 0.66 | 0.937607 |
Target: 5'- --cGCCGCgcAGGaAGGCguCGuaGAGGUCCg -3' miRNA: 3'- guaUGGCGauUCC-UCUG--GC--CUCCAGG- -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 70764 | 0.66 | 0.937607 |
Target: 5'- --gGCCGUUGGGGAccGGCUaguguuuuuGGAGGcCCu -3' miRNA: 3'- guaUGGCGAUUCCU--CUGG---------CCUCCaGG- -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 50109 | 0.66 | 0.937607 |
Target: 5'- --aGCUGCUGGGGgccGGGCUGGGGcuGUCg -3' miRNA: 3'- guaUGGCGAUUCC---UCUGGCCUC--CAGg -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 42955 | 0.66 | 0.936133 |
Target: 5'- --cGCCGCgcgcgGGGGGGGCgGGccgccggccggcccGGGUUCg -3' miRNA: 3'- guaUGGCGa----UUCCUCUGgCC--------------UCCAGG- -5' |
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6456 | 3' | -54.2 | NC_001847.1 | + | 56588 | 0.66 | 0.934636 |
Target: 5'- uGUGCCGCUGAGcGAcugcgugaucgaagaGGCCG-AGG-CCg -3' miRNA: 3'- gUAUGGCGAUUC-CU---------------CUGGCcUCCaGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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