Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6673 | 5' | -55.3 | NC_001847.1 | + | 4173 | 0.66 | 0.875343 |
Target: 5'- gGGCCUuguuuUGGGCCGCGCgccCGUGGg-- -3' miRNA: 3'- -UUGGA-----ACCUGGUGCGguuGCACCagu -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 9485 | 0.67 | 0.844055 |
Target: 5'- aAACCcaGGACCGCGCCG--GUGGa-- -3' miRNA: 3'- -UUGGaaCCUGGUGCGGUugCACCagu -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 18822 | 0.73 | 0.496716 |
Target: 5'- cGGCCUUcagcuuGACCACGUUGGCGUGGUCc -3' miRNA: 3'- -UUGGAAc-----CUGGUGCGGUUGCACCAGu -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 19893 | 0.69 | 0.743072 |
Target: 5'- cAACCggcGGAgUggGUCAGCGUGGUCGa -3' miRNA: 3'- -UUGGaa-CCUgGugCGGUUGCACCAGU- -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 20326 | 0.67 | 0.849775 |
Target: 5'- cGCCgagGGACCAuguacCGCCAgggcacccccaaccGCGUGGUg- -3' miRNA: 3'- uUGGaa-CCUGGU-----GCGGU--------------UGCACCAgu -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 20726 | 0.67 | 0.860129 |
Target: 5'- cGCUggcGGACCgcuucuaccucaACGCCGACGUGGaUCc -3' miRNA: 3'- uUGGaa-CCUGG------------UGCGGUUGCACC-AGu -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 27863 | 0.69 | 0.742079 |
Target: 5'- gGGCCcugUGGGCCGCGCUcgagcgguuggauGGCGaGGUCGc -3' miRNA: 3'- -UUGGa--ACCUGGUGCGG-------------UUGCaCCAGU- -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 30185 | 0.68 | 0.809555 |
Target: 5'- -----cGGACCugGCCGACGcGGUg- -3' miRNA: 3'- uuggaaCCUGGugCGGUUGCaCCAgu -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 30719 | 0.66 | 0.875343 |
Target: 5'- -uCC-UGGAcgcCCGCGCgGACGUGGcCGa -3' miRNA: 3'- uuGGaACCU---GGUGCGgUUGCACCaGU- -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 32987 | 0.79 | 0.259852 |
Target: 5'- gAGCUUgcggcGGGCCGCGCCGcCGUGGUCGc -3' miRNA: 3'- -UUGGAa----CCUGGUGCGGUuGCACCAGU- -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 43781 | 0.67 | 0.852196 |
Target: 5'- uGCCggugcUGGACgcgCGCGCCGGCGcgccGGUCGa -3' miRNA: 3'- uUGGa----ACCUG---GUGCGGUUGCa---CCAGU- -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 44771 | 0.66 | 0.896449 |
Target: 5'- gGGCCgUGGuCCGCGuCCAGCa-GGUCGc -3' miRNA: 3'- -UUGGaACCuGGUGC-GGUUGcaCCAGU- -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 45895 | 0.68 | 0.809555 |
Target: 5'- aAGCCgaGGACCugGCCGGucCGUcGGUa- -3' miRNA: 3'- -UUGGaaCCUGGugCGGUU--GCA-CCAgu -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 53322 | 0.67 | 0.852196 |
Target: 5'- gGACCUcgcgcUGGACCACGCCGcaGCGg----- -3' miRNA: 3'- -UUGGA-----ACCUGGUGCGGU--UGCaccagu -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 58576 | 0.66 | 0.896449 |
Target: 5'- aGAUCggGGGCCGgGCCGccggcuGCGUGGgCAg -3' miRNA: 3'- -UUGGaaCCUGGUgCGGU------UGCACCaGU- -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 59431 | 0.66 | 0.896449 |
Target: 5'- cGCCccGGGCCugGCUGACGaccaacUGGUUg -3' miRNA: 3'- uUGGaaCCUGGugCGGUUGC------ACCAGu -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 63109 | 0.67 | 0.844055 |
Target: 5'- cGCCccgGGGCCGcCGCCGcgcACG-GGUCGg -3' miRNA: 3'- uUGGaa-CCUGGU-GCGGU---UGCaCCAGU- -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 63773 | 0.66 | 0.875343 |
Target: 5'- aGGCCcgGaGACCGCGCgGACGcgggcuuucUGGUCGc -3' miRNA: 3'- -UUGGaaC-CUGGUGCGgUUGC---------ACCAGU- -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 67579 | 0.69 | 0.772369 |
Target: 5'- aGGCUUUGGcCCGCGCCGAUcUGGg-- -3' miRNA: 3'- -UUGGAACCuGGUGCGGUUGcACCagu -5' |
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6673 | 5' | -55.3 | NC_001847.1 | + | 70273 | 0.67 | 0.867847 |
Target: 5'- uACCgcgaGGACgCGCGCCuGGCGgGGUCGc -3' miRNA: 3'- uUGGaa--CCUG-GUGCGG-UUGCaCCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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