Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6676 | 5' | -58.2 | NC_001847.1 | + | 1066 | 0.69 | 0.587335 |
Target: 5'- gGCGGCgccggGCUguucgcugcUCCCCGCCUg---UGAc -3' miRNA: 3'- aCGCCGa----CGA---------AGGGGCGGGaacaACU- -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 2475 | 0.68 | 0.63859 |
Target: 5'- gGCGGCgGCgcCCCCGCCgCcgUG-UGAa -3' miRNA: 3'- aCGCCGaCGaaGGGGCGG-Ga-ACaACU- -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 13954 | 0.68 | 0.63859 |
Target: 5'- cUGCGGCUGCggCccgugggagCCCGCCCcUGg--- -3' miRNA: 3'- -ACGCCGACGaaG---------GGGCGGGaACaacu -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 16466 | 0.69 | 0.60779 |
Target: 5'- cUGCGGCccgGCccaacCCCCGCCCUgccUGGg -3' miRNA: 3'- -ACGCCGa--CGaa---GGGGCGGGAacaACU- -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 20030 | 0.67 | 0.71987 |
Target: 5'- gGCGGCccUGCggCCgCCGCCCUg----- -3' miRNA: 3'- aCGCCG--ACGaaGG-GGCGGGAacaacu -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 22824 | 0.68 | 0.679546 |
Target: 5'- gGCGGCcGC-UCCCCGCCgagguaGUUGu -3' miRNA: 3'- aCGCCGaCGaAGGGGCGGgaa---CAACu -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 31575 | 0.69 | 0.56701 |
Target: 5'- gGCGGCggGCcggCCUCGCCCUaGggGGg -3' miRNA: 3'- aCGCCGa-CGaa-GGGGCGGGAaCaaCU- -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 32111 | 0.72 | 0.441346 |
Target: 5'- cGCGGCUGCgg-CCCGCCCg------ -3' miRNA: 3'- aCGCCGACGaagGGGCGGGaacaacu -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 34603 | 0.72 | 0.423396 |
Target: 5'- cGCGGCcgGC-UCCCCGCCCa------ -3' miRNA: 3'- aCGCCGa-CGaAGGGGCGGGaacaacu -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 39183 | 0.67 | 0.699822 |
Target: 5'- aGCGGCUGCUgcgcucacgcUCgCCCGCCagcGUUc- -3' miRNA: 3'- aCGCCGACGA----------AG-GGGCGGgaaCAAcu -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 40330 | 0.68 | 0.679546 |
Target: 5'- aGCGGCUGCggCCCuCGgcgaCCUUGa--- -3' miRNA: 3'- aCGCCGACGaaGGG-GCg---GGAACaacu -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 44655 | 0.7 | 0.536883 |
Target: 5'- --aGGCUGCUgCCCCGCCUgcaccgUGAc -3' miRNA: 3'- acgCCGACGAaGGGGCGGGaaca--ACU- -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 44815 | 0.69 | 0.56701 |
Target: 5'- gGCGGCUGCgcCCCCgGCCCc------ -3' miRNA: 3'- aCGCCGACGaaGGGG-CGGGaacaacu -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 47221 | 0.69 | 0.601643 |
Target: 5'- cGCGGCcGCgcccagccccacgCCCCGCCCcUGUg-- -3' miRNA: 3'- aCGCCGaCGaa-----------GGGGCGGGaACAacu -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 47734 | 0.7 | 0.50733 |
Target: 5'- cGCGGCggGCggggCCCCGCCCg------ -3' miRNA: 3'- aCGCCGa-CGaa--GGGGCGGGaacaacu -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 57520 | 0.66 | 0.777895 |
Target: 5'- gGCGGC-GCgccgUCCUCGCCCa------ -3' miRNA: 3'- aCGCCGaCGa---AGGGGCGGGaacaacu -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 60660 | 0.66 | 0.787159 |
Target: 5'- cGCGGCcGCgUCCgCGCCCUc----- -3' miRNA: 3'- aCGCCGaCGaAGGgGCGGGAacaacu -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 62452 | 0.66 | 0.768499 |
Target: 5'- gGCGGCcGCUcUCCCCGgCCUc----- -3' miRNA: 3'- aCGCCGaCGA-AGGGGCgGGAacaacu -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 63286 | 0.66 | 0.749349 |
Target: 5'- cGCGGCcGCUUCCaCCGCU---GggGAg -3' miRNA: 3'- aCGCCGaCGAAGG-GGCGGgaaCaaCU- -5' |
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6676 | 5' | -58.2 | NC_001847.1 | + | 77096 | 0.68 | 0.683616 |
Target: 5'- cGCGaGCUGCgucagccgggucgCCagcagcgggCCGCCCUUGUUGc -3' miRNA: 3'- aCGC-CGACGaa-----------GG---------GGCGGGAACAACu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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