Results 1 - 20 of 49 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6684 | 5' | -55 | NC_001847.1 | + | 27611 | 0.65 | 0.947673 |
Target: 5'- cCCGuuAGGCgcggCCAUCGUuccucGUCCG-CUCg -3' miRNA: 3'- -GGCcuUCCGa---GGUAGCA-----CAGGUaGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 127007 | 0.65 | 0.947673 |
Target: 5'- cCCGcGAGGCU-CGUCGg--CCcgCCCg -3' miRNA: 3'- -GGCcUUCCGAgGUAGCacaGGuaGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 24194 | 0.65 | 0.947673 |
Target: 5'- cCCGcGAGGCU-CGUCGg--CCcgCCCg -3' miRNA: 3'- -GGCcUUCCGAgGUAGCacaGGuaGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 9732 | 0.65 | 0.947243 |
Target: 5'- uCCGGcggcgccGGGGCagucgCCGUCG-GUCCAcCUCg -3' miRNA: 3'- -GGCC-------UUCCGa----GGUAGCaCAGGUaGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 100835 | 0.66 | 0.938625 |
Target: 5'- cCCGGgcGGCUCCGgccagggcCGgagcgCCggCCCg -3' miRNA: 3'- -GGCCuuCCGAGGUa-------GCaca--GGuaGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 33920 | 0.66 | 0.938625 |
Target: 5'- uCgGGGAGGCUggCCucccCGUGUgCAggCCCg -3' miRNA: 3'- -GgCCUUCCGA--GGua--GCACAgGUa-GGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 80851 | 0.66 | 0.938625 |
Target: 5'- uCCGGgcGGCggcgCgCGUCGaggCCGUCCg -3' miRNA: 3'- -GGCCuuCCGa---G-GUAGCacaGGUAGGg -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 113488 | 0.66 | 0.933747 |
Target: 5'- gCCGGggGGCUCgCccuccgGUCGcGcCCGggcgcggCCCg -3' miRNA: 3'- -GGCCuuCCGAG-G------UAGCaCaGGUa------GGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 10675 | 0.66 | 0.933747 |
Target: 5'- gCCGGggGGCUCgCccuccgGUCGcGcCCGggcgcggCCCg -3' miRNA: 3'- -GGCCuuCCGAG-G------UAGCaCaGGUa------GGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 83726 | 0.66 | 0.933747 |
Target: 5'- gCGGgcGGCUCCGUC-UGcgCCG-CCg -3' miRNA: 3'- gGCCuuCCGAGGUAGcACa-GGUaGGg -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 78268 | 0.66 | 0.928631 |
Target: 5'- gCCGGcGGGC-CCGUUGg--CCGcgCCCg -3' miRNA: 3'- -GGCCuUCCGaGGUAGCacaGGUa-GGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 101883 | 0.66 | 0.928631 |
Target: 5'- ---aGGGGCUCCAUCGcGUcggCCAgCCCg -3' miRNA: 3'- ggccUUCCGAGGUAGCaCA---GGUaGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 128075 | 0.66 | 0.925447 |
Target: 5'- cCCGGgcGGCUCgCcgCGUG-CCuugguggcgaaagCCCa -3' miRNA: 3'- -GGCCuuCCGAG-GuaGCACaGGua-----------GGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 40989 | 0.66 | 0.921068 |
Target: 5'- gCCGGGccgcgcucaccuGCUCCggcGUCGUG-CCGUCCUg -3' miRNA: 3'- -GGCCUuc----------CGAGG---UAGCACaGGUAGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 55563 | 0.67 | 0.917684 |
Target: 5'- cCCGGAcGGCgCCcgcagcacgAUCGUGUUugCAUCCa -3' miRNA: 3'- -GGCCUuCCGaGG---------UAGCACAG--GUAGGg -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 82 | 0.67 | 0.917684 |
Target: 5'- cCCGGGcccgcucuGGGCUCCGccccuggGUCCGgcgCCCc -3' miRNA: 3'- -GGCCU--------UCCGAGGUagca---CAGGUa--GGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 102895 | 0.67 | 0.917684 |
Target: 5'- cCCGGGcccgcucuGGGCUCCGccccuggGUCCGgcgCCCc -3' miRNA: 3'- -GGCCU--------UCCGAGGUagca---CAGGUa--GGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 2620 | 0.67 | 0.911853 |
Target: 5'- gCCGGAGGuGCUUCggCGggaccgCCGUCgCCg -3' miRNA: 3'- -GGCCUUC-CGAGGuaGCaca---GGUAG-GG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 60995 | 0.67 | 0.911257 |
Target: 5'- aCGGcgccgucGAGGCcgCCAUCGgcgccgcgcGUCaCAUCCCc -3' miRNA: 3'- gGCC-------UUCCGa-GGUAGCa--------CAG-GUAGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 39863 | 0.67 | 0.909455 |
Target: 5'- gCCGGuAGGCUUCGUCcacgcggcccggGUCCAggCCg -3' miRNA: 3'- -GGCCuUCCGAGGUAGca----------CAGGUagGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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