Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6727 | 3' | -60.1 | NC_001875.2 | + | 88255 | 0.66 | 0.727996 |
Target: 5'- aCGGGCGuuG-GCUGG-CUUGGCUgcuGCg -3' miRNA: 3'- -GCCUGCucCaCGACCuGGACCGGu--CG- -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 128889 | 0.66 | 0.718298 |
Target: 5'- gGGGCGcGGcucgcgGCgcaGACCUGGgCGGCg -3' miRNA: 3'- gCCUGCuCCa-----CGac-CUGGACCgGUCG- -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 19346 | 0.66 | 0.718298 |
Target: 5'- uGGGCGuGGUGCgcGGAgCguUGGCCGa- -3' miRNA: 3'- gCCUGCuCCACGa-CCUgG--ACCGGUcg -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 16227 | 0.66 | 0.708529 |
Target: 5'- uCGGGCGAGuGUGaCUGcggcGACgaGGCCGucacGCg -3' miRNA: 3'- -GCCUGCUC-CAC-GAC----CUGgaCCGGU----CG- -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 109169 | 0.66 | 0.688814 |
Target: 5'- --aACGAGGUGCUcaGCCUGGUCAc- -3' miRNA: 3'- gccUGCUCCACGAccUGGACCGGUcg -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 68260 | 0.66 | 0.678883 |
Target: 5'- -cGACGuGGUGCUGcucGugUUGGCCAu- -3' miRNA: 3'- gcCUGCuCCACGAC---CugGACCGGUcg -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 117345 | 0.67 | 0.668917 |
Target: 5'- -cGACGAGGUGCcGG-CCUGcaGCCAc- -3' miRNA: 3'- gcCUGCUCCACGaCCuGGAC--CGGUcg -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 83458 | 0.67 | 0.658921 |
Target: 5'- -aGACGccGGUGCUGGGCCacgUGuGCguGCu -3' miRNA: 3'- gcCUGCu-CCACGACCUGG---AC-CGguCG- -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 72591 | 0.67 | 0.65792 |
Target: 5'- gCGGGCGcugcGGGcGCuUGGGCCUccagguuguuuacGGCCuGCg -3' miRNA: 3'- -GCCUGC----UCCaCG-ACCUGGA-------------CCGGuCG- -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 111880 | 0.67 | 0.648905 |
Target: 5'- aCGG-CGAGGcgccgGCcGGACCgugcGcGCCGGCc -3' miRNA: 3'- -GCCuGCUCCa----CGaCCUGGa---C-CGGUCG- -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 49521 | 0.67 | 0.648905 |
Target: 5'- gCGGaccGCGAccagucgcuGGUGCUGGACUUGgggcccgacGCCAGa -3' miRNA: 3'- -GCC---UGCU---------CCACGACCUGGAC---------CGGUCg -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 118661 | 0.67 | 0.647902 |
Target: 5'- uGaACGAGGUGCUgcacgccGGACacuUGGCguGCg -3' miRNA: 3'- gCcUGCUCCACGA-------CCUGg--ACCGguCG- -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 47489 | 0.67 | 0.61881 |
Target: 5'- uCGGGCGAccaaaaugcaGUGCUGGAugcauCCUcGGCgAGCg -3' miRNA: 3'- -GCCUGCUc---------CACGACCU-----GGA-CCGgUCG- -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 52461 | 0.68 | 0.608788 |
Target: 5'- -cGACGAGGaGCuguuUGGGCUguccGGCCAGUu -3' miRNA: 3'- gcCUGCUCCaCG----ACCUGGa---CCGGUCG- -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 88354 | 0.68 | 0.608788 |
Target: 5'- aCGGACGAGGUGUcguaGCUgucGGgCAGCg -3' miRNA: 3'- -GCCUGCUCCACGacc-UGGa--CCgGUCG- -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 20428 | 0.68 | 0.588801 |
Target: 5'- uGGcCGAGGUGCaGGAaaaUUGcGaCCAGCg -3' miRNA: 3'- gCCuGCUCCACGaCCUg--GAC-C-GGUCG- -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 105895 | 0.68 | 0.588801 |
Target: 5'- uGGGCGAGGaGCUcaucGACCagugGGgCAGCg -3' miRNA: 3'- gCCUGCUCCaCGAc---CUGGa---CCgGUCG- -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 98410 | 0.68 | 0.57885 |
Target: 5'- gCGGACGuuGUGUUucaagcGGACgaGcGCCAGCu -3' miRNA: 3'- -GCCUGCucCACGA------CCUGgaC-CGGUCG- -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 45743 | 0.68 | 0.568935 |
Target: 5'- cCGGAguCGGGcccGCUGGACUUGG-CGGCg -3' miRNA: 3'- -GCCU--GCUCca-CGACCUGGACCgGUCG- -5' |
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6727 | 3' | -60.1 | NC_001875.2 | + | 109415 | 0.68 | 0.568935 |
Target: 5'- cCGGGCGcGGcGCguuGGCC-GGCCGGCu -3' miRNA: 3'- -GCCUGCuCCaCGac-CUGGaCCGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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