Results 1 - 20 of 53 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6773 | 5' | -50.2 | NC_001875.2 | + | 358 | 0.68 | 0.981618 |
Target: 5'- uGUCGaucugcACG-UUGGCCGCGCGAUu- -3' miRNA: 3'- -CAGCaaa---UGCaAACUGGCGCGCUGcc -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 1054 | 0.66 | 0.99583 |
Target: 5'- -gCGUUgggcgcaACGUUggcGACCGCGCGGucaGGc -3' miRNA: 3'- caGCAAa------UGCAAa--CUGGCGCGCUg--CC- -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 2286 | 0.69 | 0.968637 |
Target: 5'- cGUCGUgaUGCGcgcgcgccgUGGCCGCGCGcagcuCGGc -3' miRNA: 3'- -CAGCAa-AUGCaa-------ACUGGCGCGCu----GCC- -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 7029 | 0.66 | 0.994339 |
Target: 5'- -aCGUaaGCGaggUGGCCGCGCGcuuguCGGc -3' miRNA: 3'- caGCAaaUGCaa-ACUGGCGCGCu----GCC- -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 9706 | 0.67 | 0.988695 |
Target: 5'- cGUCGUgcGCGcc-GGCCGCGCcGCGc -3' miRNA: 3'- -CAGCAaaUGCaaaCUGGCGCGcUGCc -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 11870 | 0.69 | 0.96541 |
Target: 5'- uGUUuUUUGCGg-UGugCGCGgCGACGGa -3' miRNA: 3'- -CAGcAAAUGCaaACugGCGC-GCUGCC- -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 14807 | 0.76 | 0.738896 |
Target: 5'- ------cACuUUUGGCCGCGCGGCGGg -3' miRNA: 3'- cagcaaaUGcAAACUGGCGCGCUGCC- -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 15003 | 0.69 | 0.974439 |
Target: 5'- --aGUUUGCGUgcugUGACUGUGcCGACa- -3' miRNA: 3'- cagCAAAUGCAa---ACUGGCGC-GCUGcc -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 16225 | 0.7 | 0.950179 |
Target: 5'- aGUCGg--GCGagugUGACUGCGgCGACGa -3' miRNA: 3'- -CAGCaaaUGCaa--ACUGGCGC-GCUGCc -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 19041 | 0.67 | 0.990965 |
Target: 5'- cUCGaUUAUGU--GGCCGCGCucgaggugaacucgGACGGg -3' miRNA: 3'- cAGCaAAUGCAaaCUGGCGCG--------------CUGCC- -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 19938 | 0.69 | 0.968637 |
Target: 5'- -cCGUacACGgagcGGCCgGCGCGACGGu -3' miRNA: 3'- caGCAaaUGCaaa-CUGG-CGCGCUGCC- -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 21062 | 0.72 | 0.90782 |
Target: 5'- gGUCGgcgGCGUUuugGGCaaaagaCGCGCGGCGGc -3' miRNA: 3'- -CAGCaaaUGCAAa--CUG------GCGCGCUGCC- -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 21739 | 0.67 | 0.991325 |
Target: 5'- -gCGUucUUGCuc-UGGCCGCGCGugGCGGc -3' miRNA: 3'- caGCA--AAUGcaaACUGGCGCGC--UGCC- -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 27694 | 0.66 | 0.994339 |
Target: 5'- aUCGcgccgcCGUUUGAgCCGCacaacgagGCGACGGg -3' miRNA: 3'- cAGCaaau--GCAAACU-GGCG--------CGCUGCC- -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 29260 | 0.7 | 0.950179 |
Target: 5'- -gCGggUGCGcggccgccGACCGCGCcGGCGGg -3' miRNA: 3'- caGCaaAUGCaaa-----CUGGCGCG-CUGCC- -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 41124 | 0.68 | 0.979417 |
Target: 5'- cGUCGUgucGCGcUUGACCG-GCGGCc- -3' miRNA: 3'- -CAGCAaa-UGCaAACUGGCgCGCUGcc -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 42192 | 0.66 | 0.995131 |
Target: 5'- gGUUGg--GCGUUUcaaauAgCGCGCGGCGGc -3' miRNA: 3'- -CAGCaaaUGCAAAc----UgGCGCGCUGCC- -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 43526 | 0.69 | 0.974439 |
Target: 5'- cGUCGguggACGUgacGACCGCGguGCGGa -3' miRNA: 3'- -CAGCaaa-UGCAaa-CUGGCGCgcUGCC- -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 46619 | 0.75 | 0.797127 |
Target: 5'- cGUCGacaUGCGgcgcuACCGCGCGGCGGc -3' miRNA: 3'- -CAGCaa-AUGCaaac-UGGCGCGCUGCC- -5' |
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6773 | 5' | -50.2 | NC_001875.2 | + | 48295 | 0.69 | 0.968637 |
Target: 5'- --aGUggGCGUguuugcGACUGCGCGACGc -3' miRNA: 3'- cagCAaaUGCAaa----CUGGCGCGCUGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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