Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6783 | 5' | -49.9 | NC_001875.2 | + | 22590 | 0.67 | 0.983796 |
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Target: 5'- gCGCGuuGGCCGGCcGGC--UGUacggUUUCGu -3' miRNA: 3'- -GCGUuuCCGGUCG-CCGuaACA----AAAGU- -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 551 | 0.67 | 0.985689 |
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Target: 5'- uGUGGAuguaGCCGGUGGCGUUGUgcaagucgUUCAa -3' miRNA: 3'- gCGUUUc---CGGUCGCCGUAACAa-------AAGU- -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 109994 | 0.67 | 0.987408 |
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Target: 5'- aCGCGc-GGUCGGUGGCGUUGa----- -3' miRNA: 3'- -GCGUuuCCGGUCGCCGUAACaaaagu -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 18621 | 0.66 | 0.988964 |
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Target: 5'- aGCAAAGGCgCAGCucGGU--UGUUgugUCGu -3' miRNA: 3'- gCGUUUCCG-GUCG--CCGuaACAAa--AGU- -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 63447 | 0.66 | 0.988964 |
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Target: 5'- gGCGucggcGGCCAGCcgcgaGGCGUUGgUUUCc -3' miRNA: 3'- gCGUuu---CCGGUCG-----CCGUAACaAAAGu -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 94082 | 0.66 | 0.990365 |
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Target: 5'- gGCGcAGGuUUAGCGGCAgguugUGUUUUUu -3' miRNA: 3'- gCGUuUCC-GGUCGCCGUa----ACAAAAGu -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 130061 | 0.66 | 0.990365 |
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Target: 5'- gGCGgcGGCggCGGCGGCAUgcucGUggUCAc -3' miRNA: 3'- gCGUuuCCG--GUCGCCGUAa---CAaaAGU- -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 73042 | 0.66 | 0.992747 |
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Target: 5'- uGCAGcuGGGCCAacuCGGCGcUGUUUUg- -3' miRNA: 3'- gCGUU--UCCGGUc--GCCGUaACAAAAgu -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 43903 | 0.66 | 0.993748 |
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Target: 5'- uGCAGAGGCggggcguuugCGGUGGCAUcugGUaUUCc -3' miRNA: 3'- gCGUUUCCG----------GUCGCCGUAa--CAaAAGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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