Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6982 | 5' | -50.7 | NC_001875.2 | + | 74371 | 0.66 | 0.99101 |
Target: 5'- -cGUGCGCGGCcgcGGcgCGuUCAGGCg- -3' miRNA: 3'- acUACGCGCUGu--UCuaGC-AGUUCGac -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 89294 | 0.66 | 0.990885 |
Target: 5'- cUGG-GCGCGACcaccgugGAGAU-GUCGAGCg- -3' miRNA: 3'- -ACUaCGCGCUG-------UUCUAgCAGUUCGac -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 108976 | 0.66 | 0.989699 |
Target: 5'- cGAUGCGCuGCAGcacggUGUCcAGGCUGg -3' miRNA: 3'- aCUACGCGcUGUUcua--GCAG-UUCGAC- -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 59479 | 0.66 | 0.988243 |
Target: 5'- --uUGCGCagcgcggccgaGACAAGcgCGUCGAGUg- -3' miRNA: 3'- acuACGCG-----------CUGUUCuaGCAGUUCGac -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 130326 | 0.66 | 0.988243 |
Target: 5'- aGA-GCGCccgcacccaaaaGGCAGGGUCGUC--GCUGa -3' miRNA: 3'- aCUaCGCG------------CUGUUCUAGCAGuuCGAC- -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 45715 | 0.66 | 0.988089 |
Target: 5'- ---cGCGCGACGaaGGGUgcuccgcCGcCGAGCUGg -3' miRNA: 3'- acuaCGCGCUGU--UCUA-------GCaGUUCGAC- -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 119301 | 0.66 | 0.986633 |
Target: 5'- gGGUGUuuGACGAGAUCGacaaaGAGUUGa -3' miRNA: 3'- aCUACGcgCUGUUCUAGCag---UUCGAC- -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 24493 | 0.66 | 0.984858 |
Target: 5'- -uGUGCGCGcaaaGCAAGAUCGgcgCAcGCg- -3' miRNA: 3'- acUACGCGC----UGUUCUAGCa--GUuCGac -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 13151 | 0.67 | 0.98291 |
Target: 5'- ---aGCGUGGCA---UCGUCGAGCa- -3' miRNA: 3'- acuaCGCGCUGUucuAGCAGUUCGac -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 62368 | 0.67 | 0.980778 |
Target: 5'- gGGUcGCGCGcCAGGGagGUCGAcauGCUGa -3' miRNA: 3'- aCUA-CGCGCuGUUCUagCAGUU---CGAC- -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 49879 | 0.67 | 0.978453 |
Target: 5'- ---gGCGCcaacCAAGugGUCGUCGAGCUGc -3' miRNA: 3'- acuaCGCGcu--GUUC--UAGCAGUUCGAC- -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 126531 | 0.68 | 0.970235 |
Target: 5'- aGGUaGCGCG-CAAuggaguGGUCGUCGGGCg- -3' miRNA: 3'- aCUA-CGCGCuGUU------CUAGCAGUUCGac -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 16237 | 0.69 | 0.951938 |
Target: 5'- gUGAcUGCgGCGACGAGGcCGUCAcGCg- -3' miRNA: 3'- -ACU-ACG-CGCUGUUCUaGCAGUuCGac -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 7888 | 0.69 | 0.951938 |
Target: 5'- ---cGcCGCGGCAGu-UUGUCAAGCUGg -3' miRNA: 3'- acuaC-GCGCUGUUcuAGCAGUUCGAC- -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 129433 | 0.69 | 0.947533 |
Target: 5'- cGGUGCGCGACA-----GUCAGGCc- -3' miRNA: 3'- aCUACGCGCUGUucuagCAGUUCGac -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 66029 | 0.69 | 0.942869 |
Target: 5'- cGA-GCGCGugGAGuacauucCGcUCAAGCUGg -3' miRNA: 3'- aCUaCGCGCugUUCua-----GC-AGUUCGAC- -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 11882 | 0.69 | 0.932755 |
Target: 5'- -uGUGCGCGGCGacggagGGGUCGUCGacaaugagcAGCUc -3' miRNA: 3'- acUACGCGCUGU------UCUAGCAGU---------UCGAc -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 79225 | 0.7 | 0.902836 |
Target: 5'- uUGuUGUGCGACGAGAUguagcUGuUUAAGCUGg -3' miRNA: 3'- -ACuACGCGCUGUUCUA-----GC-AGUUCGAC- -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 112953 | 0.71 | 0.896066 |
Target: 5'- -cGUGCGCGACAA-AUCGUCGGGa-- -3' miRNA: 3'- acUACGCGCUGUUcUAGCAGUUCgac -5' |
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6982 | 5' | -50.7 | NC_001875.2 | + | 90849 | 0.71 | 0.896066 |
Target: 5'- aGGUGCGCGACGucgucaaauucGGuGUUGUCGAacguGCUGg -3' miRNA: 3'- aCUACGCGCUGU-----------UC-UAGCAGUU----CGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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